Effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae
文献类型： 外文期刊
作者： Qin, Chuanjie 1 ; Zhao, Daxian 2 ; Gong, Quan 3 ; Qi, Zemin 1 ; Zou, Yuanchao 1 ; Yue, Xingjian 1 ; Xie, Biwen 1 ;
作者机构： 1.Neijiang Normal Univ, Coll Life Sci, Neijiang 641112, Peoples R China
2.Nanchang Univ, Sch Life Sci & Food Engn, Nanchang 330013, Jiangxi, Peoples R China
3.Sichuan Acad Agr Sci, Fisheries Inst, Chengdu 611731, Sichuan, Peoples R China
关键词： Heat shock protein 70;Botia reevesae;Ammonia-N;Aeromonas hydrophila
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： The objective of this study was to investigate the effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae. After ammonia-N exposure at a constant concentration of 7.21 +/- 0.10 mg L-1 for 96 h, B. reevesae was challenged with Aeromonas hydrophila. Quantitative PCR analysis showed predominant and significant expression of HSP70 in liver, gill, skin, spleen and kidney (P < 0.05), with significantly upregulated expression of the mRNA transcript in these tissues after sublethal ammonia-N exposure and A. hydrophila challenge. Furthermore, following A. hydrophila challenge after ammonia-N exposure, HSP70 mRNA expression was significantly upregulated in kidney and gill tissues, although its expression levels were significantly lower than those detected following A. hydrophila challenge or ammonia-N exposure individually. These results indicate that B. reevesae HSP70 is involved in resistance to pathogenic bacteria. It is hypothesized that ammonia-N results in the downregulation of HSP70 mRNA in immune organs after an A. hydrophila challenge, thus lowering their resistance to pathogenic stress. (C) 2013 Published by Elsevier Ltd.
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