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Biochemical, transcriptomic and metabolomic responses to total dissolved gas supersaturation and their underlying molecular mechanisms in Yangtze sturgeon (Acipenser dabryanus)

文献类型： 外文期刊

作者： Chen, Yeyu 1 ; Wu, Xiaoyun 1 ; Liu, Xiaoqing 2 ; Lai, Jiansheng 1 ; Liu, Ya 1 ; Song, Mingjiang 1 ; Li, Feiyang 1 ; Gong, Quan 1 ;

作者机构： 1.Fishery Inst Sichuan Acad Agr Sci, Chengdu 611730, Peoples R China

2.Xihua Univ, Key Lab Fluid & Power Machinery, Minist Educ, Chengdu 610039, Peoples R China

关键词： Acipenser dabryanus; Total dissolved gas; Oxidative response; Heat shock protein; Energy metabolism; Immunity

期刊名称：ENVIRONMENTAL RESEARCH （ 影响因子：8.431； 五年影响因子：8.399 ）

ISSN： 0013-9351

年卷期： 2023 年 216 卷

页码：

收录情况： SCI

摘要： With the rapid development of hydropower facility construction, the total dissolved gas (TDG) generated by dam discharge is seriously threatening the survival of fish and has become an ecological environmental issue of global concern. However, how TDG affects fish physiology and the underlying molecular mechanism remain poorly known. In this study, Acipenser dabryanus, an ancient living fossil that is a flagship species of the Yangtze River, was exposed to water supersaturated with TDG at a level of 116% for 48 h. A comprehensive analysis was performed to study the effect of TDG supersaturation stress on A. dabryanus, including histopathological, biochemical, transcriptomic and metabolomic analyses. The histopathological results showed that mucosal-associated lymphoid tissues were seriously damaged after TDG supersaturation stress. Plasma catalase levels increased significantly under TDG supersaturation stress, while superoxide dismutase levels decreased signifi-cantly. Transcriptomic analysis revealed 289 upregulated genes and 162 downregulated genes in gill tissue and 535 upregulated and 104 downregulated genes in liver tissue. Metabolomic analysis revealed 63 and 164 differentially abundant metabolites between the control group and TDG group in gill and liver, respectively. The majority of heat shock proteins and genes related to ubiquitin and various immune-related pathways were significantly upregulated by TDG supersaturation stress. Integrated transcriptomic and metabolomic analyses revealed the upregulation of amino acid metabolism and glycometabolism pathways under TDG supersaturation stress. Glycerophospholipid metabolism was increased which might be associated with maintaining cell mem-brane integrity. This is the first study revealing the underlying molecular mechanisms of effects of TDG super -saturation on fish. Our results suggested that acute TDG supersaturation stress could enhance immune and antioxidative functions and activate energy metabolic pathways as an adaptive mechanism in A. dabryanus.

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