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Histological, ultrastructural, and multi-omic integration analysis on the response mechanisms of Acipenser dabryanus to hypoxia and reoxygenation

文献类型： 外文期刊

作者： Chen, Yeyu 1 ; Wu, Xiaoyun 1 ; Li, Pengcheng 1 ; Lai, Jiansheng 1 ; Ni, Luyun 1 ; Liu, Zhao 1 ; Song, Mingjiang 1 ; Li, Feiyang 1 ; Gong, Quan 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Fisheries Res Inst, Chengdu 611730, Peoples R China

2.Sichuan Acad Agr Sci, Fish Resources & Environm Upper Reaches Yangtze Ri, Chengdu 611730, Peoples R China

关键词： Hypoxia; Reoxygenation; Acipenser dabryanus; Multi-omics

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2026 年 610 卷

页码：

收录情况： SCI

摘要： Hypoxia is a global issue affecting aquaculture because of increasingly serious environmental pollution. Revealing the changes in the physiological function of fish and the molecular mechanisms after hypoxic stress is key to avoiding damage from hypoxia. In this study, we systematically investigated the effects of hypoxia and reoxygenation on Acipenser dabryanus using histological, ultrastructural, biochemical, and multi-omic analyses. Histological studies showed that the skin and muscle were damaged to different degrees after hypoxia and that the tissue structure returned to normal after reoxygenation. Scanning and transmission electron microscopy revealed that the morphology of mitochondria-rich and pavement cells in the gills of the hypoxic group was significantly altered and damaged. The activities of serum superoxide dismutase and liver lactate dehydrogenase increased after hypoxia. Transcriptome sequencing of the liver and gills identified numerous differentially expressed genes (DEGs) between the hypoxia and control groups, whereas the number of DEGs in the reoxygenation versus control group was significantly reduced. DEGs enriched in cytokine-cytokine receptor interactions, the PI3K-Akt signalling pathway, the HIF-1 signalling pathway, and glycolysis/gluconeogenesis may play important roles in the response to hypoxic stress. Differentially expressed metabolites and proteins screened by metabolomics and proteomics confirmed the transcriptomic results. The combined analysis of multi-omics showed that glycolysis/gluconeogenesis metabolism was upregulated after hypoxia to increase glucose production to obtain more energy under hypoxic environment. Muti-omics analysis also revealed the enhancement of glycerophospholipid metabolism which might be a self-protection mechanism to maintain the stability of the cell membrane against hypoxic stress. This study provided meaningful insights into the molecular mechanisms underlying the effects of hypoxic stress and reoxygenation in fish.

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