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High-density genetic map construction and comparative genome analysis in asparagus bean

文献类型： 外文期刊

作者： Huang, Haitao 2 ; Tan, Huaqiang 1 ; Xu, Dongmei 2 ; Tang, Yi 1 ; Niu, Yisong 2 ; Lai, Yunsong 1 ; Tie, Manman 3 ; Li, Hua 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Hort, Chengdu 611130, Sichuan, Peoples R China

2.Mianyang Acad Agr Sci, Mianyang 621023, Peoples R China

3.Dazhou Acad Agr Sci, Dazhou 635000, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： Genetic maps are a prerequisite for quantitative trait locus (QTL) analysis, marker-assisted selection (MAS), fine gene mapping, and assembly of genome sequences. So far, several asparagus bean linkage maps have been established using various kinds of molecular markers. However, these maps were all constructed by gel-or array-based markers. No maps based on sequencing method have been reported. In this study, an NGS-based strategy, SLAF-seq, was applied to create a high-density genetic map for asparagus bean. Through SLAF library construction and Illumina sequencing of two parents and 100 F2 individuals, a total of 55,437 polymorphic SLAF markers were developed and mined for SNP markers. The map consisted of 5,225 SNP markers in 11 LGs, spanning a total distance of 1,850.81 cM, with an average distance between markers of 0.35 cM. Comparative genome analysis with four other legume species, soybean, common bean, mung bean and adzuki bean showed that asparagus bean is genetically more related to adzuki bean. The results will provide a foundation for future genomic research, such as QTL fine mapping, comparative mapping in pulses, and offer support for assembling asparagus bean genome sequence.

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