Integrated metabolome and transcriptome analysis revealed color formation in purple leaf mustard ( Brassica juncea)
文献类型： 外文期刊
作者： Di, Hongmei 1 ; Zhao, Yatian 1 ; Zhou, Aolian 1 ; Chen, Zhifeng 2 ; Ma, Jie 3 ; Liu, Duchen 4 ; Escalona, Victor Hugo 5 ; Qian, Guiping 6 ; Yu, Xuena 1 ; Huang, Huanhuan 1 ; Tang, Yi 1 ; Li, Huanxiu 1 ; Zhang, Fen 1 ; Huang, Zhi 1 ; Sun, Bo 1 ;
作者机构： 1.Sichuan Agr Univ, Coll Hort, 211 Huimin Rd, Chengdu 611130, Sichuan, Peoples R China
2.Zunyi Normal Univ, Coll Biol & Agr Technol, Zunyi 563000, Peoples R China
3.Bijie lnst Agr Sci, Bijie 551700, Peoples R China
4.Sichuan Acad Agr Sci, Hort Res Inst, Chengdu 610066, Peoples R China
5.Univ Chile, Fac Agr Sci, Santa Rosa 11315, Santiago 8820808, Metropolitan Re, Chile
6.Chengdu Jinniu Dist Ctr Dis Control & Prevent, Chengdu 610037, Peoples R China
关键词： Mustard; Flavonoids; Anthocyanin; Photosynthesis; Transcription factors
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.9； 五年影响因子：4.3 ）
ISSN： 0304-4238
年卷期： 2024 年 337 卷
页码：
收录情况： SCI
摘要： Purple leaf mustard has garnered attention owing to its abundant anthocyanin content. This study investigated the phenolic metabolome and transcriptome of purple variety ZC-Red and green variety ZC-2. Each of the two varieties had three biological replicates, and each replicate consisted of ten leaves from five plants. Phenolic metabolome was analyzed using an ultra-high performance liquid chromatography system and tandem mass spectrometry, and the transcriptome sequencing platform was the Illumina NovaSeq 6000 platform. The green variety ZC-2 was used as the control for differential analysis. All differential metabolites and genes were significant. Findings revealed the accumulation of anthocyanins in ZC-Red, whereas ZC-2 exhibited higher levels of phenolic acids and flavonoids, excluding anthocyanins. This could be attributed to the directional preference of the phenylpropanoid metabolic flow. Transcriptome analysis highlighted the pivotal roles of phenylpropanoid metabolism and photosynthesis in color formation. In ZC-Red, nearly all anthocyanin biosynthesis genes exhibited up-regulation, with multiple genes in late biosynthesis showing increases thousands of times greater than ZC-2. Conversely, the expression of genes associated with photosystem subunits and light-harvesting complexes was down-regulated. Key candidate transcription factors, including MYB113, TT8, WRKY44, and MYB30, were identified as regulators of anthocyanin and photosynthesis. Findings contribute valuable insights into the molecular mechanisms underlying anthocyanin accumulation. Core differential metabolites such as anthocyanins and phenolic acids are of great significance to the utilization of diversified functional compounds and targeted breeding of leaf mustard.
- 相关文献
作者其他论文 更多>>
-
The Preventive Effect of Theabrownin from Ya'an Tibetan Tea Against UVB-Induced Skin Photodamage in BALB/c Mice via the MAPK/NF-κB and Nrf2 Signaling Pathways
作者：Xu, Jingyi;Xie, Mingji;Luo, Peida;Yang, Xinyao;Zhao, Jing;Bian, Jinlin;Sun, Bo;Tang, Qian;Du, Xiao;Zou, Yao;He, Chunlei;Xu, Jingyi;Xie, Mingji;Luo, Peida;Yang, Xinyao;Zhao, Jing;Bian, Jinlin;Tang, Qian;Du, Xiao;Zou, Yao;He, Chunlei;Liang, Xing;Dai, Weidong
关键词：theabrownin; Tibetan tea; photodamage; antioxidant; anti-inflammation; apoptosis
-
Genome-Wide Analysis of Mlo Genes and Functional Characterization of Cm-mlo38 and Cm-mlo44 in Regulating Powdery Mildew Resistance in Melon
作者：Gong, Fangyi;Lan, Yanhong;Zhang, Tian;Li, Chun;Li, Yifan;Xia, Feng;Liu, Xiaojun;Liu, Duchen;Liang, Genyun;Cai, Peng;Fang, Chao;Gong, Fangyi;Lan, Yanhong;Zhang, Tian;Li, Chun;Li, Yifan;Xia, Feng;Liu, Xiaojun;Liu, Duchen;Liang, Genyun;Cai, Peng;Fang, Chao
关键词：
Cucurbitaceae ; powdery mildew resistance; Mlo -
Genome-wide characterization of the cytochrome P450 gene family in Solanum melongena and functional analysis of SmCYP82C1 under cold stress
作者：Lan, Yanhong;Gong, Fangyi;Liu, Xiaojun;Liu, Duchen;Liang, Genyun;Li, Chun;Xia, Feng;Li, Yifan;Fang, Chao;Cai, Peng;Lan, Yanhong;Gong, Fangyi;Liu, Xiaojun;Liu, Duchen;Liang, Genyun;Li, Chun;Xia, Feng;Li, Yifan;Fang, Chao;Cai, Peng;Lan, Yanhong;Gong, Fangyi;Liu, Xiaojun;Liu, Duchen;Liang, Genyun;Li, Chun;Xia, Feng;Li, Yifan;Fang, Chao;Cai, Peng
关键词：Genome-wide analysis;
CYP450 gene family;Solanum melongena ; Cold stress -
Variation in Nutritional Qualities and Antioxidant Capacity of Different Leaf Mustard Cultivars
作者：Cheng, Wenjuan;Luo, Anqi;Li, Hongxia;Zhang, Fen;Huang, Zhi;Tang, Yi;Li, Huanxiu;Sun, Bo;Liang, Kehao;Yu, Xuena;Cheng, Wenjuan;Chen, Zhifeng;Guan, Zhongrong;Escalona, Victor Hugo;Liu, Duchen;Zhou, Yu'nong
关键词：
Brassica juncea ; nutritional quality; variant; antioxidants; membership function -
Re-Sequencing the Mitochondrial Genome Unveils a Novel Isomeric Form of NWB CMS Line in Radish and Functional Verification of Its Candidate Sterile Gene
作者：Li, Xiaomei;Liang, Le;Tang, Yi;Li, Huanxiu;Li, Xiaomei;Yang, Feng;Ran, Maolin;Yong, Xiaoping;Kong, Chuibao;Li, Xiaomei;Yang, Feng;Ran, Jianzhao
关键词：radish; cytoplasmic male sterility; NWB CMS; mitochondrial genome; orf463a; transgenic plants
-
Mutual intercropping affects the growth and selenium content of cherry tomato seedlings
作者：Liang, Le;Li, Huanxiu;Zhou, Ailing;Tang, Wanjia;Dong, Yiping;Wang, Liming;Sun, Bo;Lin, Lijin;Sun, Guochao;Huang, Zhi;Tang, Yi;Liao, Jichao;Li, Xiaomei;Li, Xiaomei;Tu, Lihua
关键词：Cherry tomato; growth; intercropping; seedlings; selenium
-
New insights into the evolution analysis of trihelix gene family in eggplant (Solanum melongena L.) and expression analysis under abiotic stress
作者：Lan, Yanhong;Gong, Fangyi;Li, Chun;Xia, Feng;Li, Yifan;Liu, Xiaojun;Liu, Duchen;Liang, Genyun;Fang, Chao;Cai, Peng;Lan, Yanhong;Gong, Fangyi;Li, Chun;Xia, Feng;Li, Yifan;Liu, Xiaojun;Liu, Duchen;Liang, Genyun;Fang, Chao;Cai, Peng
关键词：
Solanum melongena ;Trihelix gene family; Transcription factor; Phylogenetic analysis; Abiotic stress; Expression profile
