Multi-omics analysis provides preliminary insights into the mechanisms involved in dormancy and germination in asparagus (Asparagus officinalis)
文献类型： 外文期刊
作者： Zhang, Yinchao 1 ; Sheng, Yuzhen 1 ; Lai, Jia 1 ; Liu, Yong 1 ; Zhang, Qianfang 1 ; Liu, Jia 1 ; Shi, Xiaoli 1 ; Huang, Haitao 1 ; Ye, Peng sheng 1 ; Wei, Shugu 1 ; Huang, Ling 1 ;
作者机构： 1.Sichuan Acad Agr Sci, Ind Crops Res Inst, Chengdu 610300, Peoples R China
2.Sichuan Res Ctr Vegetable Engn & Technol, Chengdu 610300, Peoples R China
3.Sichuan Prov Engn Technol Res Ctr Vegetables, Pengzhou 611930, Sichuan, Peoples R China
4.Hort Crops Germplasm Innovat & Utilizat Key Lab Si, Chengdu 610300, Peoples R China
关键词： Asparagus dormancy and germination; Integrated omics; Hormone signaling; Phenylpropanoid biosynthesis; Jasmonate pathway
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN： 0304-4238
年卷期： 2025 年 350 卷
页码：
收录情况： SCI
摘要： Dormancy and germination are critical physiological processes in asparagus (Asparagus officinalis) bud development, yet their molecular regulatory networks remain poorly understood. Here, we integrated transcriptomic and metabolomic analyses to characterize gene expression and metabolic dynamics during these stages. Principal component analysis (PCA) of 12 samples revealed distinct clustering of three biological replicates for dormancy and germination. Transcriptome analysis identified 2510 common differentially expressed genes (DEGs) during dormancy release and sprouting in asparagus rhizomes. Among these, 92 transcription factors (TFs) were significantly regulated, dominated by ZF-HD (19 members), MYB (18), and WRKY (13). Gene ontology analysis (GO) enrichment highlighted microtubule-based processes, carbohydrate metabolism, and photosynthesis, while kyoto encyclopedia of genes and genomes (KEGG) analysis revealed significant pathways in phenylpropanoid biosynthesis and starch/sucrose metabolism. Metabolomic profiling detected 331 (GS vs DS) and 173 (GR vs DR) differential metabolites, with 93 shared metabolites. Integrative analysis via Procrustes (M2 = 0.1828, p-value = 0.001) and O2PLS identified four key candidate genes (AoAHG1, AoGA3ox2, AoHB13, AoPRH132) linking transcriptional and metabolic regulation. These findings unravel potential molecular mechanisms underlying dormancy regulation and germination activation, highlighting TF-hormone-signaling crosstalk and key metabolites in asparagus bud development. We also identified two common KEGG pathways in the transcriptome and metabolome, flavonoid biosynthesis (ko00941) and starch/sucrose metabolism (ko00500), suggesting that these two pathways play important roles in dormancy and germination. This study provides a comprehensive framework for dissecting the regulatory networks governing perennial plant dormancy and sprouting, offering targets for agricultural optimization of asparagus production.
- 相关文献
作者其他论文 更多>>
-
Characterization of rhizosphere bacterial communities in oilseed rape cultivars with different susceptibility to Plasmodiophora brassicae infection
作者：Deng, Yue;Huang, Xiaoqing;Yang, Xiaoxiang;Yu, Yaoyin;Zhang, Zhongmei;Hu, Zijin;Zhou, Xiquan;Liu, Yong;Zhang, Lei;Deng, Yue;Wu, Wenxian;Huang, Xiaoqing;Yang, Xiaoxiang;Yu, Yaoyin;Zhang, Zhongmei;Liu, Yong;Zhang, Lei;Zhou, Kang;Zhou, Kang
关键词：rhizosphere microbiome;
Plasmodiophora brassicae ; susceptible cultivar; resistant cultivar; microbial metabolism; nitrogen cycle -
Transcriptome-hormone network analysis tentatively reveals a chalcone synthase 1-dependent pathway for tobacco mosaic virus resistance in tobacco
作者：Li, Fuxin;Wei, Fengjie;Ren, Min;Qu, Xiaoling;Tong, Ying;Wu, Xiuming;Sun, Yangyang;Yang, Aiguo;Chen, Shuai;Zhang, Yinchao;Li, Lihua;Wu, Jiang
关键词：Nicotiana tabacum L.; Tobacco mosaic virus; RNA-Seq; Phytohormone; WGCNA; Hub gene
-
Integrated multi-omics preliminarily reveals sex-biased gene networks controlling dormancy release in Asparagus officinalis
作者：Zhang, Yinchao;Zhang, Qianfang;Lai, Jia;Liu, Yong;Sheng, Yuzhen;Liu, Jia;Ye, Peng sheng;Wei, Shugu;Huang, Ling;Li, Xia;Ye, Peng sheng;Wei, Shugu;Zhang, Yinchao;Lai, Jia;Ye, Peng sheng;Wei, Shugu;Huang, Ling
关键词：Asparagus officinalis; Germination; Dormancy; RNA-seq; Proteome; Hub gene
-
Integrative transcriptomic and HS-SPME-GC-MS analysis reveals the influence mechanism of different altitude ecotypes on pulp aroma components and volatile metabolites in strawberry hybrid F1 and its parents
作者：He, Ruyu;Chen, Lijuan;Wang, Dong;Zhang, Guowei;Liu, Jia;Li, Hongwen;He, Ruyu;Chen, Lijuan;Wang, Dong;Zhang, Guowei;Liu, Jia;Li, Hongwen
关键词：Cultivated strawberry; Fruit quality; HS-SPME-GC-MS; Transcriptomics; Candidate genes
-
ORANGE proteins mediate adaptation to high light and resistance to Pseudomonas syringae in tomato by regulating chlorophylls and carotenoids accumulation
作者：Gao, Yongfeng;Zhou, Xue;Wang, Cheng;Xiao, Xiangxia;Wu, Jiamin;Zhou, Shan;de Dios, Victor Resco;Rodriguez, Lucas Gutierrez;Yao, Yinan;Liu, Jikai;Deng, Heng;Huang, Haitao;Wen, Jing;de Dios, Victor Resco;de Dios, Victor Resco
关键词：ORANGE protein; Photosynthetic pigments; Photooxidative stress; Pathogen resistance
-
WGCNA revealed a comprehensive gene co-expression network associated with brown spot in Nicotiana tabacum L
作者：Shen, Zhan;Jiang, Zipeng;Bai, Yalin;Jiang, Caihong;Cheng, Lirui;Wang, Yuanying;Yang, Aiguo;Liu, Dan;Zhang, Yinchao;Li, Yangyang;Qiao, Chan
关键词：Nicotiana tabacum L .; Tobacco brown spot; RNA-Seq; WGCNA; Hub gene
-
Comparative Metagenomic Analysis Reveals Rhizosphere Microbiome Assembly and Functional Adaptation Changes Caused by Clubroot Disease in Chinese Cabbage
作者：Liu, Yong;Lai, Jia;Sun, Xiaofang;Huang, Ling;Sheng, Yuzhen;Zhang, Qianfang;Zeng, Hualan;Zhang, Yinchao;Ye, Pengsheng;Wei, Shugu
关键词：Chinese cabbage; clubroot disease; rhizosphere microbiome; metagenomic sequencing
