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Re-Sequencing the Mitochondrial Genome Unveils a Novel Isomeric Form of NWB CMS Line in Radish and Functional Verification of Its Candidate Sterile Gene

文献类型： 外文期刊

作者： Li, Xiaomei 1 ; Liang, Le 1 ; Ran, Jianzhao 4 ; Yang, Feng 2 ; Ran, Maolin 2 ; Yong, Xiaoping 2 ; Kong, Chuibao 2 ; Tang, Yi 1 ; Li, Huanxiu 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Hort, Chengdu 611130, Peoples R China

2.Sichuan Acad Agr Sci, Rice & Sorghum Res Inst, Deyang Branch, Deyang 618099, Peoples R China

3.Sichuan Acad Agr Sci, Vegetable Germplasm Innovat & Variety Improvement, Chengdu 610066, Peoples R China

4.Hexi Univ, Coll Agr & Ecol Engn, Zhangye 734000, Peoples R China

关键词： radish; cytoplasmic male sterility; NWB CMS; mitochondrial genome; orf463a; transgenic plants

期刊名称：HORTICULTURAE （ 影响因子：3.1； 五年影响因子：3.4 ）

ISSN：

年卷期： 2024 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Radish (Raphanus sativus L.) is a globally significant vegetable and relies on cytoplasmic male sterile (CMS) lines for hybrid seed production. The NWB CMS type is favored over Ogura CMS for its ease in maintainer screening. Despite its varied mitochondrial configurations and unvalidated sterile gene, we re-sequenced the mitochondrial genome of NWB CMS Tibet A and verified the function of the sterility gene via genetic transformation of Arabidopsis thaliana. The mitochondrial genomes of Tibet A could be assembled into circular DNA molecules, with a mitochondrial genome size of 239,184 bp. Our analysis indicated that the specific orf463a was the CMS-associated gene in Tibet A, sharing sequence consistency with the CMS gene in DCGMS and NWB CMS YB-A. Collinearity analysis showed that the mitochondrial genomes of NWB CMS Tibet A, DCGMS, and NWB CMS YB-A share the same mitotype, with structural variations due to recombination via a 9731 bp long repeat sequence and a 508 bp short repeat sequence. Driven by the Ap3 promoter, transgenic Arabidopsis with orf463a exhibited male sterility, confirming the gene's potential role in CMS. In this study, we assembled a new isomeric form of NWB CMS mitochondrial genome and proved the function of the candidate sterile gene.

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