Purification and Identification of Novel Antioxidant Peptides from Hydrolysates of Peanuts (Arachis hypogaea) and Their Neuroprotective Activities
文献类型： 外文期刊
作者： Ma, Rui 1 ; Bai, Jinrong 1 ; Huang, Yuting 3 ; Wang, Zhiqiu 1 ; Xu, Yongju 3 ; Huang, Yan 1 ; Zhong, Kai 3 ; Huang, Yina 1 ; Gao, Hong 3 ; Bu, Qian 1 ;
作者机构： 1.Sichuan Univ, West China Hosp 4, Inst Syst Epidemiol, West China Sch Publ Hlth,Mol Toxicol Lab Sichuan P, Chengdu 610041, Peoples R China
2.Chengdu Univ Tradit Chinese Med, State Key Lab Southwestern Chinese Med Resources, Chengdu 611137, Peoples R China
3.Sichuan Univ, Coll Biomass & Engn & Hlth Food, Evaluat Res Ctr, Dept Food Sci & Technol, Chengdu 610065, Peoples R China
4.Sichuan Acad Agr Sci, Ind Crops Res Inst, Chengdu 610300, Peoples R China
关键词： Arachis hypogaea; peanut peptides; antioxidant activity; neuroprotection; PC12 cells
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2023 年 71 卷 15 期
页码：
收录情况： SCI
摘要： Peanut (Arachis hypogaea) peptides have various functional activities and a high utilization value. This study aims to isolate and characterize antioxidant peptides from peanut protein hydrolysates and further evaluate their neuroprotection against oxidative damage to PC12 cells induced by 6-hydroxydopamine (6-OHDA). After the peanut protein was hydrolyzed with pepsin and purified using ultrafiltration and gel chromatography, six peptides were identified and sequenced by high-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Out of these six peptides, Pro-Gly-Cys-Pro-Ser-Thr (PGCPST) exhibited a desirable antioxidant capacity, as determined using the 1,1-diphenyl-2-picrylhydrazyl, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, and hydroxyl radical scavenging assays. Moreover, our results indicated that the peptide PGCPST effectively increased the cell viability and reduced the cell apoptosis in 6-OHDA-induced PC12. RNA sequencing further showed that the neuroprotective effect of the peptide PGCPST was mediated via sphingolipid metabolism-related pathways. With further research efforts, the peptide PGCPST was expected to develop into a new neuroprotective agent.
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