Preparation of a thermosensitive and antibacterial in situ gel using poloxamer-quaternized chitosan for sustained ocular delivery of Levofloxacin hydrochloride
文献类型： 外文期刊
作者： Chen, Zihan 1 ; Wang, Anyu 1 ; Qin, Yiming 1 ; Chen, Xu 2 ; Feng, Xiao 3 ; He, Guangyun 4 ; Zhu, Xiaoming 2 ; Xiao, Ying 1 ; Yu, Xi 1 ; Zhong, Tian 1 ; Zhang, Kang 1 ;
作者机构： 1.Macau Univ Sci & Technol, Fac Med, Ave Wai Long, Taipa, Macau, Peoples R China
2.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa, Macau, Peoples R China
3.Nanjing Univ Finance & Econ, Coll Food Sci & Engn, Collaborat Innovat Ctr Modern Grain Circulat & Saf, Key Lab Grains & Oils Qual Control & Proc, Nanjing, Peoples R China
4.Sichuan Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Chengdu, Peoples R China
关键词： In situ gel; Quaternized chitosan; Thermosensitive; Sustained release; Antibacterial
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2024 年 283 卷
页码：
收录情况： SCI
摘要： In this study, a thermosensitive in situ gel with porous structure was developed using poloxamer (Po) and N-(2hydroxy-3-trimethyl ammonium) propyl chitosan chloride (HTCC). The poloxamer-quaternized chitosan (PoHTCC) in situ gel exhibited superior rheological property, water absorption capacity and antibacterial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus pyogenes, making it wellsuited for ocular applications. Scanning electron microscope revealed a macroporous architecture with pore sizes ranging from 1 to 2 mu m, suggesting that the gel has desirable breathability, corneal adhesion capability, and overall conformability. In vitro drug release assay was conducted with levofloxacin hydrochloride, demonstrating that sustained release over 48 h could be achieved at 34 degrees C, with approximately 80 % of the drug released within this timeframe. Computational simulations revealed substantial binding affinity between the material and the Escherichia coli outer membrane lipopolysaccharide-associated protein and corneal mucin. The protein showing the strongest binding energy to N-(2-hydroxy-3-trimethyl ammonium) propyl chitosan chloride (HTCC), as calculated by the Molecular Mechanics Generalized Born Surface Area Method (MM-GBSA), was LptD-LptE, with a binding energy of -61.14 +/- 4.72 kcal/mol. These results underscore the potential of this system for effective and convenient ocular delivery with sustained drug release.
- 相关文献
作者其他论文 更多>>
-
A preliminary study of combined toxicity and underlying mechanisms of imidacloprid and cadmium coexposure using a multiomics integration approach
作者：Wang, Yuankai;Zhuang, Ziyue;Shi, Hui;Du, Muying;Kan, Jianquan;Chen, Kewei;Cai, Tian;Wang, Yuankai;Zhuang, Ziyue;Shi, Hui;Du, Muying;Kan, Jianquan;Cai, Tian;Chen, Kewei;Zalan, Zsolt;Wang, Yuankai;Zhuang, Ziyue;Shi, Hui;Du, Muying;Kan, Jianquan;Chen, Kewei;Wang, Yuankai;Zhuang, Ziyue;Shi, Hui;Du, Muying;Kan, Jianquan;Chen, Kewei;He, Guangyun
关键词：Imidacloprid; Cadmium; Combined toxicity; Untargeted metabolomics; Transcriptomics; Oxidative stress
-
Magnetic-gold nanoparticle-mediated paper-based biosensor for highly sensitive colorimetric detection of food adulteration
作者：Wang, Anyu;Chen, Zihan;Zhong, Tian;Xiao, Ying;Yu, Xi;Feng, Xiao;He, Guangyun
关键词：Small-scale equipment; Visualized detection; Colorimetric reaction; DNA enrichment; Digital analysis
-
Multi-pesticide residue screening, identification, and quantification analysis in various fruits and vegetables by UHPLC-Q Exactive HRMS
作者：Wang, Fengyi;Jiao, Ying;Qiu, Shiting;Han, Mei;Hou, Xue;He, Guangyun;Qin, Shudi;Wang, Fengyi;Jiao, Ying;Qiu, Shiting;Han, Mei;Hou, Xue;He, Guangyun;Qin, Shudi
关键词：
-
Using bioactive compounds to mitigate the formation of typical chemical contaminants generated during the thermal processing of different food matrices
作者：Guo, Zilong;Yang, Huanqi;Zhong, Tian;Xiao, Ying;Yu, Xi;Feng, Xiao;He, Guangyun
关键词：bioactive compounds; chemical contaminants; inhibition; mechanisms; thermal processing
-
Safety risk of using asomate to reduce acid in Citrus production
作者：He, Guangyun;Hou, Xue;Qiu, Shiting;Qin, Shudi;Wang, Fengyi;He, Guangyun;Hou, Xue;Qiu, Shiting;Qin, Shudi;Wang, Fengyi;Xie, Rong;Yu, Xi;Yu, Xi;Chen, Xi
关键词：Citrus; Sweetness; Asomate; Organic acid; Safety
-
Silk fibroin microneedles loaded with epigallocatechin gallate mitigate atrazine-induced testicular toxicity
作者：Fang, Chunyan;He, Guangyun;Luo, Fan;Hou, Xue;Ye, Yulong;Li, Yuchen;Gan, Ren-You;Lei, Lanjie
关键词：Atrazine; Epigallocatechin gallate; EGCG-SF-MNs; Apoptosis; Necroptosis
-
UPLC-Q-TOF/MS-based metabolomic analysis reveals the effects of asomate on the citrus fruit
作者：He, Guangyun;Hou, Xue;Han, Mei;Qiu, Shiting;Li, Ying;Qin, Shudi;Wang, Fengyi;He, Guangyun;Hou, Xue;Han, Mei;Qiu, Shiting;Li, Ying;Qin, Shudi;Wang, Fengyi;Chen, Xi;Yu, Xi
关键词：Citrus fruits; Citric acid; Asomate; UPLC-Q-TOF/MS; Metabolomics; Chemometrics
