文献类型： 外文期刊

作者： Xia, Chen ¹ ; Zhao, Sijia ¹ ; Yu, Manyou ¹ ; Deng, Junlin ¹ ; Xiang, Zhuoya ¹ ; Chen, Jian ¹ ; Zhang, Ting ³ ; Tang, Xiaobo ¹ ; Zhu, Yongqing ¹ ; Jin, Yan ⁴ ; Zeng, Ruiqi ² ; Zhao, Jiayuan ² ; Shi, Liugang ³ ; Wu, Bin ⁵ ;

作者机构： 1.Sichuan Acad Agr Sci, Inst Food Nutr & Hlth, Inst Agroprod Proc Sci & Technol, Chengdu 610066, Peoples R China

2.Sichuan Normal Univ, Coll Life Sci, Chengdu 610100, Sichuan, Peoples R China

3.Sichuan Acad Agr Sci, Tea Res Inst, Chengdu 610066, Peoples R China

4.Tianjin Univ Sci & Technol, Coll Food Sci & Engn, Tianjin 300457, Peoples R China

5.Yazhou Hengtai Tea Ind Co LTD, Yaan 625100, Sichuan, Peoples R China

关键词： Golden flower Tibetan tea polysaccharides (GFTPS); Ultrasonic modification; Antioxidant activity; In vitro digestion; Short-chain fatty acids (SCFAs); Prebiotic potential

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.6； 五年影响因子：6.9 ）

ISSN： 0023-6438

年卷期： 2025 年 230 卷

页码：

收录情况： SCI

摘要： This study systematically investigated the impact of ultrasonic treatment (120-200 W) on the structural and functional properties of Golden Flower Tibetan Tea Polysaccharides (GFTPS), focusing on their antioxidant capacity, digestibility, and prebiotic potential. The results showed that ultrasonication induced significant molecular weight reduction from 16,036.94 Da to 10,642.35 Da at 200 W and increased uronic acid content up to 274.28 mg/g, which may owe to the cavitation-driven cleavage of glycosidic bonds and polysaccharide-protein dissociation. Compared to untreated sample, the antioxidant activities of GFTPS were maximized by 160 W treatment, with the half maximal inhibitory concentration (IC50) values of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging decreasing by 10.2 % and 18.8 %, respectively. Meanwhile, alpha-glucosidase inhibition of GFTPS was enhanced at 200 W treatment with IC50 of 77.16 mu g/mL, which might link to improved enzyme accessibility of low-MW fragments. During in vitro simulated digestion, ultrasonication modified GFTPS at 200 W (UGFTPS) exhibited higher resistance to enzymatic degradation, retaining 276.89 mg/g uronic acid post-intestinal phase. Colonic fermentation of UGFTPS elevated short-chain fatty acids (SCFAs) production and enriched Bacteroides, Lachnospira and Klebsiella populations, correlating with pH reduction and butyrate accumulation (7.63 mg/g at 48 h). Functional annotation revealed the carbohydrate metabolism pathways and SCFA biosynthesis might be upregulated. These results demonstrate that optimal ultrasonication at 200 W optimizes GFTPS bioactivity by balancing structural modification and stability, positioning ultrasound as a green strategy to engineer polysaccharides for nutraceutical applications.