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Introgression of a novel Thinopyrum intermedium St-chromosome-specific HMW-GS gene into wheat

文献类型： 外文期刊

作者： Li, Guang-Rong 1 ; Liu, Cheng 1 ; Li, Cheng-Hui 1 ; Zhao, Jin-Mei 1 ; Zhou, Li 1 ; Dai, Gang 1 ; Yang, En-Nian 2 ; Yang, 1 ;

作者机构： 1.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Peoples R China

2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China

关键词： High-molecular-weight glutenin subunit;In-situ hybridization;Thinopyrum intermedium ssp trichophorum;Wheat quality improvement

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2013 年 31 卷 4 期

页码：

收录情况： SCI

摘要： Thinopyrum intermedium has been hybridized extensively with wheat (Triticum aestivum L.) and several genes for disease resistance have been introgressed to cultivated wheat. However, there are very few reports about the Th. intermedium-derived seed storage protein genes which have been transferred into a wheat background by chromosome manipulation. Our aim is to identify several wheat-Th. intermedium ssp. trichophorum derivatives, and document these lines by genomic in situ hybridization (GISH), molecular markers and seed storage protein analysis. We found that a novel Th. intermedium 1St#2 chromosome-specific high-molecular-weight glutenin subunit (HMW-GS) was transferred to the wheat-Thinopyrum derivative lines. The genomic sequence of the Thinopyrum-derived HMW-GS was characterized and designated Glu-1St#2x, since it resembled x-type glutenins in both the N-terminal domain and C-terminal domain. It is much shorter than that of reported HMW-GS genes. The Glu-1St#2x sequence was successfully expressed in Escherichia coli and resulted in the identical weight to the native protein. The GISH and newly developed chromosome Thinopyrum-specific DNA markers enabled physically location of Glu-1St#2x to the region FL0.60-1.00 on Th. intermedium 1St#2L chromosome arm. Phylogenetic analysis revealed that the Glu-1St#2x evolved earlier than other x-type HMW-GS homoeologues in modern wheat genomes. The effect of Glu-1St#2x on protein content, sodium dodecyl sulphate sedimentation value and improvement of solvent retention capacity in wheat background suggested that Th. intermedium chromosome 1St#2 may have potential for improvement of wheat end-product quality.

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