您好，欢迎访问四川省农业科学院 机构知识库！

Isolation of a new repetitive DNA sequence from Secale africanum enables targeting of Secale chromatin in wheat background

文献类型： 外文期刊

作者： Liu, Cheng 1 ; Yang, Zu-Jun 1 ; Li, Guang-Rong 1 ; Zeng, Zi-Xian 1 ; Zhang, Yong 2 ; Zhou, Jian-Ping; Liu, Zhao-Hui;

作者机构： 1.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Peoples R China; Sichuan Agr Univ, Key Lab Plant Genet & Breeding, Yaan 625014, Peoples R China; Sichuan Acad Agr Sci, Ind Crop Breeding & Cultivat Inst, Jianyang 641400, Peoples R China

2.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Peoples R China; Sichuan Agr Univ, Key Lab Plant Genet & Breeding, Yaan 625014, Peo

关键词： Secale africanum;rye;molecular marker;FISH;chromatin detection

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2008 年 159 卷 1-2 期

页码：

收录情况： SCI

摘要： A genome specific DNA sequence that detects Secale africanum chromatin incorporated into wheat was developed in this study. Random amplified polymorphic DNA (RAPD) analysis was used to search for genome specific DNA sequences of S. africanum in lines, R111, "mianyang11" (MY11) and wheat-rye 1RS/1BL translocations R25 and R57. A high copy rye-specific DNA segment pSaD15(940) of the S. africanum genome was obtained. The sequence of pSaD15 did not show any significant homology to other reported sequences in databases and it is therefore a new repetitive sequence of Secale. PCR primers were designed for pSaD15(940), which amplify a clear 887 bp fragment in S. africanum but not in any wheat. The primers also amplified an 887 bp fragment in other accessions of rye, Chinese Spring-Imperial rye chromosome additions and a diverse range of material carrying different rye chromosomes or chromosomal segments. In situ hybridization showed that probe pSaD15(940) was specifically hybridized throughout all rye chromosomes arms except for the terminal regions. The advantage of the rye-specific probe developed herein compared to those of previous reports is that it has been shown to be widely applicable to other Secale species. The probe will be useful as a molecular marker for the introgression of S. africanum and other rye chromosome segments into the wheat genome.

  • 相关文献

[1]Characterization of wheat-Secale africanum chromosome 5R(a) derivatives carrying Secale specific genes for grain hardness. Gao, Dan,La, Shixiao,Wang, Hongjin,Li, Jianbo,He, Weilin,Yang, Zujun,Yang, Ennian.

[2]Isolation of two new retrotransposon sequences and development of molecular and cytological markers for Dasypyrum villosum (L.). Jiang, Yun,Guo, Yuanlin,Deng, Guangbing,Yu, Maoqun,Long, Hai,Xuan, Pu,Zhang, Jie.

[3]Gene Discovery in Triticum dicoccoides, the Direct Progenitor of Cultivated Wheats. Sun, D. F.,Peng, Y. L.,Nevo, E.,Peng, J. H.. 2013

[4]Genetic Diversity Revealed by Single Nucleotide Polymorphism Markers in a Worldwide Germplasm Collection of Durum Wheat. Sun, Daokun,Chen, Liang,Peng, Junhua,Ren, Jing,You, Frank M.,Wang, Jirui,Luo, Ming-Cheng,You, Frank M.,Peng, Yunliang,Nevo, Eviatar,Sun, Dongfa,Peng, Junhua. 2013

[5]Identification of SNPs and development of allelic specific PCR markers for high molecular weight glutenin subunit D(t)x1.5 from Aegilops tauschii through sequence characterization. Yang, WY,Zhang, WJ,Lu, BR. 2005

[6]Fine mapping of a pistilloid-stamen (PS) gene on the short arm of chromosome 1 in rice. Li, Yunfeng,Yang, Zhenglin,Zhong, Bingqiang,Xie, Rong,Ren, Maozhi,Luo, Da,He, Guanghua. 2006

[7]Identification of two novel waxy alleles and development of their molecular markers in sorghum. Li, Yan,Fan, Jing,Zhao, Jiqun,Xu, Yongju,Wang, Wenming,Zhao, Ganlin,Ding, Guoxiang,Ni, Xianlin.

[8]Molecular detection of rye (Secale cereale L.) chromatin in wheat line 07jian126 (Triticum aestivum L.) and its association to wheat powdery mildew resistance. Long, Hai,Zhang, Jie,Deng, Guangbing,Pan, Zhifen,Yu, Maoqun,Yu, Shuiyang,Zhang, Erliang,Yang, Hong,Zhang, Jie.

[9]Association mapping of stigma and spikelet characteristics in rice (Oryza sativa L.). Li, Yong,Luo, Dagang,Gao, Fangyuan,Lu, Xianjun,Ren, Guangjun,Agrama, Hesham A..

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：四川省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台