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Quantification of ochratoxin A in red wines by conventional HPLC-FLD using a column packed with core-shell particles

文献类型： 外文期刊

作者： Mao, Jianfei 2 ; Lei, Shaorong 1 ; Yang, Xiaofeng 1 ; Xiao, Dan 3 ;

作者机构： 1.Sichuan Acad Agr Sci, Anal & Testing Ctr, Chengdu 610066, Peoples R China

2.Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Chengd, Chengdu 610066, Peoples R China

3.Sichuan Univ, Coll Chem, Chengdu 610065, Peoples R China

4.Sichuan Univ, Coll Chem Engn, Chengdu 610065, Peoples R China

关键词： HPLC;FLD;OTA;Core-shell column;Red wine

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2013 年 32 卷 2 期

页码：

收录情况： SCI

摘要： Ochratoxin A (OTA) is a mycotoxin commonly present in red wine and other foodstuffs. It is widely studied due to its nephrotoxic, immunotoxic, teratogenic and carcinogenic effects. Nowadays, liquid chromatography is the main method for OTA analysis. With the recent development on the column technology, core-shell columns were commercialized in recent years. It is with high performance while maintain low back pressure thus could be used on conventional HPLC instrument. However, as current conventional benchmark high-performance liquid chromatographs were not initially designed for the recently developed high-efficiency packed columns, the HPLC-FLD parameter should be carefully investigated. In this work the performance of chromatography column packed with 2.6 pm core-shell particles on conventional benchmark HPLC were investigated. Parameters including flow rate, mobile phase composition (volume fraction of ACN in water), temperature, response time, sampling frequency, and injection volume for quantitative analysis of OTA in red wines were investigated in detail. The results show that core-shell column is more effective and faster to be operated on conventional HPLC than other total porous particle packed column. Optimized conditions provided a method for the separation of OTA in less than 5 min, with the limit of detection (LOD) 0.0025 mu g/L and the limit of quantification (LOQ) 0.0083 mu g/L in the sample, respectively. The developed method was validated with red wine samples with OTA concentrations ranging from 0.0028 to 0.0437 mu g/L. The use of a core-shell column allows highly efficient, sensitive, and accurate determination of OTA with an outstanding sample throughout. (C) 2013 Elsevier Ltd. All rights reserved.

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