您好，欢迎访问四川省农业科学院 机构知识库！

Quantification of ochratoxin A in red wines by conventional HPLC-FLD using a column packed with core-shell particles

文献类型： 外文期刊

作者： Mao, Jianfei 2 ; Lei, Shaorong 1 ; Yang, Xiaofeng 1 ; Xiao, Dan 3 ;

作者机构： 1.Sichuan Acad Agr Sci, Anal & Testing Ctr, Chengdu 610066, Peoples R China

2.Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Chengd, Chengdu 610066, Peoples R China

3.Sichuan Univ, Coll Chem, Chengdu 610065, Peoples R China

4.Sichuan Univ, Coll Chem Engn, Chengdu 610065, Peoples R China

关键词： HPLC;FLD;OTA;Core-shell column;Red wine

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2013 年 32 卷 2 期

页码：

收录情况： SCI

摘要： Ochratoxin A (OTA) is a mycotoxin commonly present in red wine and other foodstuffs. It is widely studied due to its nephrotoxic, immunotoxic, teratogenic and carcinogenic effects. Nowadays, liquid chromatography is the main method for OTA analysis. With the recent development on the column technology, core-shell columns were commercialized in recent years. It is with high performance while maintain low back pressure thus could be used on conventional HPLC instrument. However, as current conventional benchmark high-performance liquid chromatographs were not initially designed for the recently developed high-efficiency packed columns, the HPLC-FLD parameter should be carefully investigated. In this work the performance of chromatography column packed with 2.6 pm core-shell particles on conventional benchmark HPLC were investigated. Parameters including flow rate, mobile phase composition (volume fraction of ACN in water), temperature, response time, sampling frequency, and injection volume for quantitative analysis of OTA in red wines were investigated in detail. The results show that core-shell column is more effective and faster to be operated on conventional HPLC than other total porous particle packed column. Optimized conditions provided a method for the separation of OTA in less than 5 min, with the limit of detection (LOD) 0.0025 mu g/L and the limit of quantification (LOQ) 0.0083 mu g/L in the sample, respectively. The developed method was validated with red wine samples with OTA concentrations ranging from 0.0028 to 0.0437 mu g/L. The use of a core-shell column allows highly efficient, sensitive, and accurate determination of OTA with an outstanding sample throughout. (C) 2013 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Quantification of aflatoxin M1 in raw milk by a core-shell column on a conventional HPLC with large volume injection and step gradient elution. Lei, Shaorong,Liu, Yunhua,Fu, Chengping,Zhong, Lingli,Ouyang, Huaxue,Mao, Jianfei,Lei, Shaorong,Fu, Chengping,Zhong, Lingli,Ouyang, Huaxue,Xiao, Dan,Xiao, Dan.

[2]不同灵芝主要活性成分差异性分析. 甘炳成,张小平. 2010

[3]HPLC法测定大黄药材和饮片中番泻苷A和番泻苷B的含量. 李敏,杨小多,于素玲,黄先鸣. 2008

[4]麻疯树种子中佛波酯的提取及对菜青虫的杀虫活性. 李静,李莉,卢代华,陈放. 2011

[5]高效液相色谱法测定蓝莓叶中绿原酸的含量. 陈建,夏陈,邓俊琳,李艳琳,李娟. 2017

[6]HPLC法测定南瓜中β-胡萝卜素. 朱永清,刘小俊,杨宏,梁根云,陈建,夏陈,张盈娇,李娟. 2017

[7]AccQ·Tag^TM法测定鱼粉水解液中的氨基酸. 韩梅. 1999

[8]Rapid determination of momordicoside in Momoradica charantia seeds by capillary zone electrophoresis. Sun, Qun,Chen, Fang,Xuan, Pu,Chen, Xin,Liang, Jia-bei.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：四川省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台