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Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility

文献类型： 外文期刊

作者： Jiao, Zhixin 1 ; Zhu, Xinxin 1 ; Li, Huijuan 1 ; Liu, Zhitao 2 ; Huang, Xinyi 2 ; Wu, Nan 2 ; An, Junhang 1 ; Li, Junchan 1 ;

作者机构： 1.Henan Agr Univ, Natl Ctr Engn & Technol Res Wheat, Natl Key Lab Wheat & Maize Crop Sci, Zhengzhou, Henan, Peoples R China

2.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China

3.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu, Sichuan, Peoples R China

关键词： Wheat (Triticum aestivum L.); dms; Chromosome; Nullisomic line; Pollen development

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2020 年 8 卷

页码：

收录情况： SCI

摘要： A dwarf, multi-pistil and male sterile dms mutant was previously reported by us. However, the genetic changes in this dms are unclear. To examine the genetic changes, single nucleotide polymorphism (SNP) association, chromosome counting, and high-resolution chromosome fluorescence in situ hybridization (FISH) techniques were employed. By comparing tall plants (T) with dwarf plants (D) in the offspring of dms mutant plants, SNP association analysis indicated that most SNPs were on chromosome 2A. There were three types in offspring of dms plants, with 42, 41 and 40 chromosomes respectively. High-resolution chromosome painting analysis demonstrated that T plants had all 42 wheat chromosomes; the medium plants (M) had 41 chromosomes, lacking one chromosome 2A; while D plants had 40 wheat chromosomes, and lacked both 2A chromosomes. These data demonstrated that dms resulted from a loss of chromosome 2A. We identified 23 genes on chromosome 2A which might be involved in the development of stamens or pollen grains. These results lay a solid foundation for further analysis of the molecular mechanisms of wheat male sterility. Because D plants can be used as a female parent to cross with other wheat genotypes, dms is a unique germplasm for any functional study of chromosome 2A and wheat breeding specifically targeting genes on 2A.

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