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Diversity and biocontrol potential of endophytic fungi in Brassica napus

文献类型： 外文期刊

作者： Zhang, Qinghua 2 ; Zhang, Jing 1 ; Yang, Long 1 ; Zhang, Lei 3 ; Jiang, Daohong 1 ; Chen, Weidong 4 ; Li, Guoqing 1 ;

作者机构： 1.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Peoples R China

3.Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu 610066, Peoples R China

4.Washington State Univ, USDA ARS, Pullman, WA 99164 USA

关键词： Brassica napus;Endophytic fungi;Volatile organic compounds;Sclerotinia sclerotiorum;Botrytis cinerea;Biological control

期刊名称：BIOLOGICAL CONTROL （ 影响因子：3.687； 五年影响因子：3.962 ）

ISSN： 1049-9644

年卷期： 2014 年 72 卷

页码：

收录情况： SCI

摘要： This study was conducted to isolate endophytic fungi from oilseed rape (Brassica napus), to identify the fungal endophytes based on morphology and ITS (ITS1-5.8S rDNA-ITS2) sequences, and to evaluate their efficacy in suppression of the plant pathogenic fungi Sclerotinia sclerotiorum and Botrytis cinerea. Selected endophytic fungal isolates were further tested for promoting growth of oilseed rape in potting experiments. A total of 97 endophytic fungal isolates were obtained from roots (35), stems (49) and leaves (13) of B. napus. Forty fungal species were identified and most species (80%) belong to Ascomycota. The species composition is highly diversified with Simpson's diversity index reaching 0.959. Alternaria alternata is the dominant species accounting for 12.4% of the isolates. Twenty-four isolates exhibited antifungal activity against S. sclerotiorum in dual cultures on potato dextrose agar forming inhibition zones of 3-17 mm in width. The culture filtrates of Aspergillus flavipes CanS-34A, Chaetomium globosum CanS-73, Clonostachys rosea CanS-43 and Leptosphaeria biglobosa CanS-51 in potato dextrose broth exhibited consistent and effective suppression of oilseed rape leaf blight caused by S. sclerotiorum. Fusarium oxysporum CanR-46 was detected capable of production of volatile organic compounds highly inhibitory to S. sclerotiorum and B. cinerea. Moreover, A. alternata CanL-18, Fusarium tricinctum CanR-70 and CanR-71r, and L. biglobosa CanS-51 exhibited growth-promoting effects on oilseed rape. These results suggest that B. napus harbors diversified endophytic fungi, from which potential biocontrol agents against S. sclerotiorum and B. cinerea, and for promoting growth of B. napus can be screened. (C) 2014 Elsevier Inc. All rights reserved.

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