文献类型： 外文期刊

作者： Song, Shipeng ¹ ; Liu, Hui ¹ ; Miao, Luke ² ; He, Li ³ ; Xie, Wenzhao ² ; Lan, Hong ¹ ; Yu, Changxiu ¹ ; Yan, Wenkai ⁶ ; Wu, Yufeng ⁶ ; Wen, Xiao-peng ⁷ ; Xu, Qiang ¹ ; Deng, Xiuxin ¹ ; Chen, Chunli ¹ ;

作者机构： 1.Hubei Hongshan Lab, Natl Key Lab Germplasm Innovat & Utilizat Fruit &, Wuhan 430070, Hubei, Peoples R China

2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China

3.Sichuan Acad Agr Sci, Hort Inst, Natl local Joint Engn Lab Citrus Breeding & Cultiv, Chengdu 610066, Sichuan, Peoples R China

4.Huazhong Agr Univ, Natl Key Lab Crop Genet & Improvement, Wuhan 430070, Hubei, Peoples R China

5.Jianghan Univ, Coll Life Sci, Hubei Prov Engn Res Ctr Legume Plants, Wuhan 430056, Hubei, Peoples R China

6.Nanjing Agr Univ, Acad Adv Interdisciplinary Studies, Bioinformat Ctr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

7.Guizhou Univ, Inst Agrobioengn, Key Lab Plant Resource Conservat & Germplasm Innov, Coll Life Sci,Minist Educ, Guiyang 550025, Guizhou, Peoples R China

关键词： Chromosomal marker; FISH; CMA banding; Genomic heterozygosity; Chromosomal translocation; Sweet orange

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：5.9； 五年影响因子：5.9 ）

ISSN： 1673-8527

年卷期： 2023 年 50 卷 6 期

页码：

收录情况： SCI

摘要： Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridi-zation (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 se-quences of FISH including centromere regions, which enables us to visualize significant differences be-tween homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the trans-location regions of chromosomes 4 and 9 in C. sinensis "Valencia." The high-resolution molecular cyto-genetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus.Copyright & COPY; 2023, The Authors. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).