您好，欢迎访问四川省农业科学院 机构知识库！

Development of homozygous tetraploid potato and whole genome doubling-induced the enrichment of H3K27ac and potentially enhanced resistance to cold-induced sweetening in tubers

文献类型： 外文期刊

作者： Guo, Hongwei 1 ; Zhou, Min 1 ; Zhang, Guoyan 1 ; He, Li 4 ; Yan, Caihong 1 ; Wan, Min 1 ; Hu, Jianjun 3 ; He, Wei 3 ; Zeng, Deying 1 ; Zhu, Bo 1 ; Zeng, Zixian 1 ;

作者机构： 1.Sichuan Normal Univ, Coll Life Sci, Dept Biol Sci, Chengdu 610101, Sichuan, Peoples R China

2.Sichuan Normal Univ, Plant Funct Genom & Bioinformat Res Ctr, Chengdu 610101, Sichuan, Peoples R China

3.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China

4.Sichuan Acad Agr Sci, Hort Inst, Chengdu 610066, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.7； 五年影响因子：9.0 ）

ISSN： 2662-6810

年卷期： 2023 年 10 卷 3 期

页码：

收录情况： SCI

摘要： Polyploid plants typically display advantages on some agronomically important traits over their diploid counterparts. Extensive studies have shown genetic, transcriptomic, and epigenetic dynamics upon polyploidization in multiple plant species. However, few studies have unveiled those alternations imposed only by ploidy level, without any interference from heterozygosity. Cultivated potato is highly heterozygous. Thus, in this study, we developed two homozygous autotetraploid lines and one homozygous diploid line in parallel from a homozygous diploid potato. We confirmed their ploidy levels using chloroplast counting and karyotyping. Oligo-FISH and genome re-sequencing validated that these potato lines are nearly homozygous. We investigated variations in phenotypes, transcription, and histone modifications between two ploidies. Both autotetraploid lines produced larger but fewer tubers than the diploid line. Interestingly, each autotetraploid line displayed ploidy-related differential expression for various genes. We also discovered a genome-wide enrichment of H3K27ac in genic regions upon whole-genome doubling (WGD). However, such enrichment was not associated with the differential gene expression between two ploidies. The tetraploid lines may exhibit better resistance to cold-induced sweetening (CIS) than the diploid line in tubers, potentially regulated through the expression of CIS-related key genes, which seems to be associated with the levels of H3K4me3 in cold-stored tubers. These findings will help to understand the impacts of autotetraploidization on dynamics of phenotypes, transcription, and histone modifications, as well as on CIS-related genes in response to cold storage.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：四川省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台