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Development and characterisation of EST-SSR markers by transcriptome sequencing in taro (Colocasia esculenta (L.) Schoot)

文献类型: 外文期刊

作者: You, Yongning 1 ; Liu, Duchen 2 ; Liu, Huabo 3 ; Zheng, Xingfei 1 ; Diao, Ying 1 ; Huang, Xinfang 4 ; Hu, Zhongli 1 ;

作者机构: 1.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Hubei, Peoples R China

2.Sichuan Acad Agr Sci, Inst Hort, Vegetable Germplasm Innovat & Variety Improvement, Chengdu 610066, Sichuan, Peoples R China

3.Hubei Engn Univ, Coll Agr, Hubei Key Lab Qual Control Characterist Fruits &, Xiaogan 432000, Hubei, Peoples R China

4.Wuhan Vegetable Inst Sci, Wuhan Natl Germplasm Repository Aquat Vegetables, Wuhan 430065, Hubei, Peoples R China

关键词: Taro;Simple sequence repeat marker;Transcriptome;Genetic diversity

期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Taro (Colocasia esculenta) is an important crop with a long history of cultivation. In this study 5278 SSRs were identified in taro transcriptome data. A total of 2858 primer pairs were designed for marker development. 100 primers were randomly selected and synthesized. Among them, 72 primer pairs were successfully amplified and 62 were polymorphic in taro accessions. The number of alleles ranged from 2 to 14 for each different polymorphic locus and the polymorphism information content valued ranged from 0.01 to 0.82. The phylogenetic tree was also constructed to analyse the genetic diversity in 68 taro accessions. The large number of taro SSR markers developed in the present study will be useful in the researches of genetic diversity, germplasm characterization and molecular breeding etc.

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