文献类型： 外文期刊

作者： Yuan, Shuai ¹ ; Yong, Xiaoping ² ; Lu, Yuxin ¹ ; Lei, Yuxin ¹ ; Li, Weijian ¹ ; Shi, Qiuli ¹ ; Yao, Xiuhong ¹ ;

作者机构： 1.Yibin Univ, Dept Agr Forestry & Food Engn, Solid State Fermentat Resource Utilizat Key Lab Si, Yibin 644000, Peoples R China

2.Sichuan Acad Agr Sci, Rice & Sorghum Res Inst, Deyang Branch, Deyang 618099, Peoples R China

3.Sichuan Acad Agr Sci, Sichuan Prov Engn Technol Res Ctr Vegetables, Vegetable Germplasm Innovat & Variety Improvement, Chengdu 610000, Peoples R China

关键词： heat stress; BrNAC19; transcriptional regulation; ROS scavenging

期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN：

年卷期： 2024 年 10 卷 12 期

页码：

收录情况： SCI

摘要： The frequent occurrence of excessive heat events driven by global warming poses a great threat to plant growth and food security. To survive in heat stress (HS) environments, plants have evolved sophisticated response mechanisms, and the transcriptional network that controls the expression levels of HS-inducible genes serves as an essential component of this process. NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play key regulatory roles in the abiotic stress responses of plants. However, the functional roles of NAC TFs in the heat stress response of Chinese cabbage remain elusive. In the present study, we identified the Brassica rapa NAC family transcription factor BrNAC19 as a close homologue of Arabidopsis NAC019 and found that it serves as a positive regulator of the HS response. BrNAC19 displayed inducible gene expression in response to HS, and its subcellular localization showed that it was localized in the nucleus. Heterologous expression of BrNAC19 significantly enhanced the heat tolerance of plants and reduced the accumulation of reactive oxygen species (ROS) under HS conditions. Furthermore, our results demonstrated that BrNAC19 directly targeted and promoted the expression of superoxide dismutase 1 (CSD1) and catalase 2 (CAT2), two antioxidant-enzyme coding genes in Chinese cabbage. Altogether, our results suggest that BrNAC19 enhances heat stress tolerance by positively regulating the expression of genes involved in the HS response and ROS scavenging and exhibits potential as a target gene in Chinese cabbage breeding to increase heat stress tolerance.