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Fine mapping of the novel male-sterile mutant gene ms39 in maize originated from outer space flight

文献类型： 外文期刊

作者： Zhu, Yonghui 2 ; Shi, Ziwen 1 ; Li, Shizhao 1 ; Liu, Heyang 1 ; Liu, Fuxia 1 ; Niu, Qunkai 1 ; Li, Chuan 1 ; Wang, Jing 1 ;

作者机构： 1.Sichuan Agr Univ, Maize Res Inst, Chengdu 611130, Sichuan, Peoples R China

2.Minist Agr, Key Lab Biol & Genet Improvement Maize Southwest, Chengdu, Sichuan, Peoples R China

3.Univ Wisconsin Madison, Dept Agron, Madison, WI 53706 USA

4.Sichuan Acad Agr Sci, Chengdu 610066, Peoples R China

5.Silkworm Res Inst Hetian, Hetian 848000, Peoples R China

6.Huaiyin Normal Univ, Huaiyin 223300, Peoples R China

关键词： Male sterility 39; Fine mapping; Callose synthase; Maize

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2018 年 38 卷 10 期

页码：

收录情况： SCI

摘要： A novel male-sterile maize mutant male sterility 39 (ms39) was obtained from offspring of the commercial hybrid Chuandan No. 9 that had been carried into outer space. A previous study demonstrated that ms39 is controlled by a single recessive nuclear gene, located on the long arm of chromosome 3. Here, we used 1073 mutant individuals derived from the (ms39xMo17) F-2 population and sequentially developed new primers to identify markers supporting the fine mapping of ms39. A 365-kb region on chromosome 3 flanked by markers L8 and M30 at a genetic distance of 0.18 and 0.47cM, respectively, was identified. According to the reference sequence of ZmB73_Ref-Gen_v4, 12 candidate genes were identified within the 365-kb mapping region. Based on cloning and sequence BLAST analysis of the 12 candidate genes, a four-base-pair deletion was found within the exon of Zm00001d043909, which encoded callose synthase12. This four-base-pair deletion resulted in a frameshift mutation in ms39, leading to the earlier termination of the coding protein, and ultimately caused abnormal performance of the callose synthase. Additionally, cytological observation was conducted on a sister cross population (ms39/ms39xms39/Ms39). These observations showed that the tapetum cells of the ms39 mutant appeared abnormal from the dyad stage, and aborted microspores were observed during pollen development. These results lay the foundation for the cloning of ms39 and exploration of the molecular mechanism underlying aborted pollen development in ms39 maize.

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