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Multi-omics analysis reveals the physiological and molecular response to cold stress in different spring wheat cultivars at the booting stage

文献类型： 外文期刊

作者： Liu, Miao 1 ; Wu, Xiaoli 1 ; Li, Ming 1 ; Li, Shizhao 1 ; Xiong, Tao 1 ; Li, Chaosu 1 ; Tang, Yonglu 1 ;

作者机构： 1.Minist Agr & Rural Affairs, Crop Res Inst Sichuan Acad Agr Sci, Crop Germplasm Innovat & Genet Improvement Key Lab, Key Lab Wheat Biol & Genet Improvement Southwester, Chengdu, Peoples R China

2.Sichuan Prov Key Lab Water Saving Agr Hill Areas S, Chengdu, Peoples R China

3.Minist Agr, Key Lab Crop Ecophysiol & Farming Syst Southwest C, Chengdu, Peoples R China

关键词： wheat; cold stress; physiological indicators; transcriptome; proteome; metabolome

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Introduction Cold stress at the booting stage can seriously affect wheat growth, development and yield.Methods Therefore, this study employed integrated physiological, transcriptomic, proteomic and metabolomic approaches to examine the response of two wheat cultivars, Chuanmai 104 (CM104, cold-tolerant) and Chuanmai 42 (CM42, cold-sensitive), to cold stress at the booting stage.Results The viability of pollen in CM104 was less affected by low-temperature stress compared to CM42, ensuring a higher seed-setting rate in CM104. The young spike of CM104 also synthesized more osmoregulatory substances, endogenous hormones and higher antioxidant enzyme activities under the cold treatment compared to CM42. Transcriptome analysis identified 7,362 and 5,328 differentially expressed genes (DEGs) between control and cold-treated CM104 and CM42 spike samples, respectively. More DEGs, such as transcription factors, late embryogenesis abundant protein and hormone signalling transduction involved in the key regulatory pathways associated with cold tolerance were expressed in CM104. Proteomic and metabolomic analyses identified 173 differentially expressed proteins and 180 differentially accumulated metabolites between control and cold-treated CM104 spike samples, with some thought to enhance the cold acclimation of the variety. Integrative multi-omics analysis highlighted the critical roles of starch and sucrose, and glycerophospholipid metabolism in response to cold stress in CM104.Discussion This study uncovered the physiological changes, gene, protein and metabolite pathways involved in maintaining the osmotic balance and mitigating low-temperature stress in wheat spikes, and could serve as a crucial reference for selecting and breeding low-temperature tolerant wheat varieties.

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