文献类型： 外文期刊

作者： Liang, Ruyun ¹ ; Tan, Luna ¹ ; Guo, Xiang ¹ ; Lou, Shangling ¹ ; Dan, Xuming ¹ ; Han, Yu ¹ ; Zeng, Cheng ¹ ; Zhang, Han ¹ ; Yang, Kai ¹ ; Chen, Liyang ¹ ; Liang, Xin ¹ ; Liu, Meng ¹ ; Guo, Mengyun ¹ ; Yin, Kangqun ¹ ; Tang, Si ¹ ; Song, Yan ¹ ; Gao, Xuemeng ¹ ; Gu, Shaobo ¹ ; Hou, Jing ¹ ; Yao, Yingjun ¹ ; Zhang, Ruijia ¹ ; Yan, Jin ¹ ; Fu, Wensen ¹ ; Li, Xuerui ¹ ; Hu, Yongqi ¹ ; Liu, Yao ¹ ; Liu, Wei ¹ ; Wu, Qiusai ¹ ; Yan, Zhen ¹ ; Jia, Weitao ⁴ ; Hu, Binhua ³ ; Wang, Jing ¹ ; Liu, Jianquan ¹ ; Liu, Huanhuan ¹ ;

作者机构： 1.Sichuan Univ, Minist Educ, Sichuan Zoige Alpine Wetland Ecosyst Natl Observat, Key Lab Bioresources & Ecoenvironm,Coll Life Sci, Chengdu 610065, Peoples R China

2.Sichuan Univ, State Key Lab Hydraul & Mt River Engn, Sichuan Zoige Alpine Wetland Ecosyst Natl Observat, Key Lab Bioresource & Ecoenvironm,Minist Educ,Coll, Chengdu 610065, Peoples R China

3.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol, Chengdu 610066, Sichuan, Peoples R China

4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China

关键词： WRKY22; RAP2.12; WRKY70; ARRs; natural variation; submergence tolerance

期刊名称：MOLECULAR PLANT （ 影响因子：24.1； 五年影响因子：25.8 ）

ISSN： 1674-2052

年卷期： 2025 年 18 卷 1 期

页码：

收录情况： SCI

摘要： Submergence stress tolerance is a complex trait governed by multiple loci. Because of its wide distribution across arid and humid regions, Arabidopsis thaliana offers an opportunity to explore the genetic components and their action mechanisms underlying plant adaptation to submergence stress. In this study, using map-based cloning we identified WRKY22 that activates RAP2.12, a locus previously identified to contribute to the submergence stress response, to regulate plant humid adaptation possibly through ethylene signal transduction in Arabidopsis. WRKY22 expression is inhibited by ARABIDOPSIS RESPONSE REGULATORs (ARRs) but activated by the WRKY70 transcription factor. In accessions from humid environments, a two-nucleotide deletion in the WRKY22 promoter region prevents binding of phosphorylated ARRs, thereby maintaining its high expression. Loss of the ARR-binding element in the WRKY22 promoter underwent strong positive selection during colonization of A. thaliana in the humid Yangtze River basin. However, the WRKY70-binding motif in the WRKY22 promoter shows no variation between accessions from humid and arid regions. These findings together establish a novel signaling axis wherein WRKY22 plays a key role in regulating the adaptive response that enables A. thaliana to colonize contrasting habitats. Notably, we further showed functional conservation of this locus in Brassica napus, suggesting that modulating this axis might be useful in the breeding of flood-tolerant crop varieties.