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Chromosome painting in meiosis reveals pairing of specific chromosomes in polyploid Solanum species

文献类型： 外文期刊

作者： He, Li 1 ; Braz, Guilherme T. 1 ; Torres, Giovana A. 4 ; Jiang, Jiming 1 ;

作者机构： 1.Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA

2.Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA

3.Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA

4.Univ Fed Lavras, Dept Biol, BR-37200 Lavras, MG, Brazil

关键词： Polyploids; Chromosome pairing; Meiosis; Chromosome painting

期刊名称：CHROMOSOMA （ 影响因子：4.316； 五年影响因子：4.159 ）

ISSN： 0009-5915

年卷期： 2018 年 127 卷 4 期

页码：

收录情况： SCI

摘要： Analysis of chromosome pairing has been an important tool to assess the genetic similarity of homologous and homoeologous chromosomes in polyploids. However, it is technically challenging to monitor the pairing of specific chromosomes in polyploid species, especially for plant species with a large number of small chromosomes. We developed oligonucleotide-based painting probes for four different potato chromosomes. We demonstrate that these probes are robust enough to monitor a single chromosome throughout the prophase I of meiosis in polyploid Solanum species. Cultivated potato (Solanum tuberosum, 2n=4x=48) is an autotetraploid. We demonstrate that the four copies of each potato chromosome pair as a quadrivalent in 66-78% of the meiotic cells at the pachytene stage. Solanum demissum (2n=6x=72) is a hexaploid and has been controversial regarding its nature as an autopolyploid or allopolyploid. Interestingly, no hexavalent pairing was observed in meiosis. Instead, we observed three independent bivalents in 83-98% of the meiotic cells at late diakinesis and early metaphase I for the four chromosomes. These results suggest that S. demissum has evolved into a cytologically stable state with predominantly bivalent pairing in meiosis.

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