Gonadal transcriptome sequencing of the critically endangered Acipenser dabryanus to discover candidate sex-related genes
文献类型： 外文期刊
作者： Chen, Yeyu 1 ; Liu, Ya 1 ; Gong, Quan 1 ; Lai, Jiansheng 1 ; Song, Mingjiang 1 ; Du, Jun 1 ; Deng, Xiaochuan 1 ;
作者机构： 1.Sichuan Acad Agr Sci, Fishery Inst, Chengdu, Sichuan, Peoples R China
关键词： Transcriptome; Differentially expressed genes; Sex-related genes; Acipenser dabryanus; Ovary; Testis
期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）
ISSN： 2167-8359
年卷期： 2018 年 6 卷
页码：
收录情况： SCI
摘要： Background: Acipenser dabryanus, an endemic Chinese species, has been listed as a first-class protected animal in China. Sturgeons are among the oldest and most primitive group of existing fish in the world and occupy a special place in the evolutionary history of fish. Thus, a study of the reproduction and sex differentiation of sturgeon will be of great value for fish as well as the whole vertebrate group. Methods: In this study, we conducted comparative analysis of the testes and ovaries transcriptomes of A. dabryanus to screen for sex-differentiation and sexual development-related genes. Results: The transcriptome sequencing of six cDNA libraries generated 265 million clean reads, encompassing 79 Gb of sequences. The N50 and mean length of the identified 91,375 unigenes were 1,718 and 989 bp, respectively. A total of 6,306, 9,961, 13,170, 15,484, and 23,588 unigenes were annotated in the clusters of orthologous groups, gene ontology categories, Kyoto Encyclopedia of Genes and Genomes Pathway, euKaryotic orthologous groups, and NCBI non-redundant protein databases, respectively. A total of 5,396 differentially expressed genes were found between the two sexes, with 1,938 predicted to be up-regulated in ovaries and 3,458 in testes. A total of 73 candidate genes known to be involved in sex differentiation and sexual development were searched in the transcriptome of A. dabryanus of which 52 showed significant similarity. We highlighted six genes that are differentially expressed between the two sexes and may play important roles in sex differentiation and gonad maintenance. In addition, 24,271 simple sequence repeats (SSRs) and 550,519 single-nucleotide polymorphisms (SNPs) were detected. Discussion: This work represents the first transcriptome study comparing the ovary and testis in A. dabryanus. The putative differentially expressed genes between the gonads provide an important source of information for further study of the sex-differentiation related genes and the sex-differentiation mechanism in sturgeons. The SSRs or SNPs identified in this study will be helpful in the discovery of sex-related markers in A. dabryanus.
- 相关文献
作者其他论文 更多>>
-
Histological, ultrastructural, and multi-omic integration analysis on the response mechanisms of Acipenser dabryanus to hypoxia and reoxygenation
作者：Chen, Yeyu;Wu, Xiaoyun;Li, Pengcheng;Lai, Jiansheng;Ni, Luyun;Liu, Zhao;Song, Mingjiang;Li, Feiyang;Gong, Quan;Chen, Yeyu;Wu, Xiaoyun;Li, Pengcheng;Lai, Jiansheng;Ni, Luyun;Liu, Zhao;Song, Mingjiang;Li, Feiyang;Gong, Quan
关键词：Hypoxia; Reoxygenation; Acipenser dabryanus; Multi-omics
-
Cloning, Expression and Stability Analysis of Candidate Reference Genes in the Glacial Relict Hucho bleekeri Under Immune Stress
作者：Wu, Xiaoyun;Lai, Jiansheng;Chen, Yeyu;Yang, Huanchao;Chen, Yanling;Liu, Zhao;Tu, Quanyu;Yu, Yi;Li, Hua;Wu, Xiaoyun;Lai, Jiansheng;Chen, Yeyu;Yang, Huanchao;Chen, Yanling;Liu, Zhao;Tu, Quanyu;Yu, Yi;Li, Hua
关键词：qPCR; RefFinder; Salmonidae; tissue distribution
-
Identification of Hybrid Sturgeon (Acipenser baerii x Acipenser schrenckii) from Their Parents Using Germplasm
作者：Yang, Shiyong;Zhao, Zhongmeng;Xu, Zihan;Jing, Zhaoxin;Pang, Xiaojian;Shao, Wuyuntana;Zhang, Chaoyang;Zhao, Zhongmeng;Liu, Ya;Jiang, Menghao;Fu, Lin;Zhang, Jin;Li, Yunkun;Du, Xiaogang;Wu, Jiayun
关键词：sturgeons; hybrids; identification; mitochondrial; microsatellites
-
Early brain gene network alterations and growth disruptions in juvenile Chinese longsnout catfish (Leiocassis longirostris) induced by 17(3-estradiol and 17α-methyltestosterone
作者：Feng, Yang;Deng, Yongqiang;Zhao, Zhongmeng;Zhao, Han;Liu, Senyue;Zhang, Lu;Duan, Yuanliang;Huang, Zhipeng;Du, Jun;Li, Qiang;Zhou, Jian;Mou, Chengyan;Ye, Hua
关键词：17(3-estradiol; 17 alpha-methyltestosterone; Chinese longsnout catfish ( Leiocassis; Brain; Immediate early genes
-
Gene-metabolite correlations underpin the hepatic fibrosis and metabolic disruption induced by methyltestosterone in juvenile Chinese longsnout catfish (Leiocassis longirostris)
作者：Feng, Yang;Deng, Yongqiang;Li, Zhao;Zhao, Zhongmeng;Zhao, Han;Liu, Senyue;Zhang, Lu;Duan, Yuanliang;Huang, Zhipeng;Du, Jun;Li, Qiang;Zhou, Jian;Mou, Chengyan;Wei, Zheng
关键词：Methyltestosterone; Chinese longsnout catfish(Leiocassis longirostris); Liver toxicity; Fibrosis; Lipid metabolism disruption; Multi-omics integration
-
Exploring the adsorption sites and mechanism of biochar towards tetracycline using machine learning
作者：Xian, Bo;Li, Qingzhi;Zhao, Huayi;Gong, Quan;Xian, Bo;Li, Qingzhi;Zhao, Huayi;Gong, Quan
关键词：Machine learning; Biochar; Tetracycline; Adsorption mechanism; Feature selection
-
The Pilot Study on the Histological and Ultrastructural Characteristics of Major Immune Organs (Spleen, Head Kidney, and Trunk Kidney) and Analysis of Pathological Features in Sichuan Taimen (Hucho bleekeri)
作者：Wei, Qinyao;Li, Hua;Chen, Yeyu;Liu, Zhao;Chen, Yanling;Yu, Yi;Tu, Quanyu;Liu, Guiliang;Du, Jun;Yang, Huanchao;Wei, Qinyao;Song, Zhaobin;Li, Hua;Chen, Yeyu;Liu, Zhao;Chen, Yanling;Yu, Yi;Tu, Quanyu;Liu, Guiliang;Du, Jun;Yang, Huanchao
关键词：head kidney; histology;
Hucho bleekeri
