Cloning, Expression and Stability Analysis of Candidate Reference Genes in the Glacial Relict Hucho bleekeri Under Immune Stress
文献类型： 外文期刊
作者： Wu, Xiaoyun 1 ; Lai, Jiansheng 1 ; Chen, Yeyu 1 ; Yang, Huanchao 1 ; Chen, Yanling 1 ; Liu, Zhao 1 ; Tu, Quanyu 1 ; Yu, Yi 1 ; Li, Hua 1 ;
作者机构： 1.Sichuan Acad Agr Sci, Fisheries Res Inst, Sichuan Fisheries Res Inst, Chengdu 611730, Peoples R China
2.Fish Resources & Environm Upper Reaches Yangtze Ri, Chengdu 611730, Peoples R China
关键词： qPCR; RefFinder; Salmonidae; tissue distribution
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.7； 五年影响因子：1.0 ）
ISSN： 0175-8659
年卷期： 2025 年 2025 卷 1 期
页码：
收录情况： SCI
摘要： To find reference genes (RGs) and establish a screening method for RGs in Sichuan taimen (Hucho bleekeri), six candidate RGs-beta-actin, b2m, beta-tubulin, ef1 alpha, gapdh and hprt - were cloned, and the stability of expression of the six candidate RGs were analysed. The six RG sequences in H. Bleekeri had higher similarity with the corresponding sequences in Salmo or Oncorhynchus. The RGs geomean of ranking values of gene expression in tissues of H. bleekeri was ef1 alpha > beta-actin > beta-tubulin > hprt > b2m > gapdh. After lipopolysaccharide (LPS) treatment, the RGs geomean of ranking values of gene expression in spleen was ef1 alpha > beta-actin > b2m > gapdh > beta-tubulin > hprt, in head kidney was beta-actin > ef1 alpha > beta-tubulin > hprt > gapdh > b2m, in liver was beta-tubulin > hprt > beta-actin > gapdh > ef1 alpha > b2m; after polyinosinic: polycytidylic acid (Poly (I:C)) treatment, the values in spleen was ef1 alpha > hprt > beta-actin > b2m > beta-tubulin > gapdh, in head kidney was beta-actin > ef1 alpha > beta-tubulin > hprt > gapdh > b2m, in liver was beta-tubulin > beta-actin > ef1 alpha > gapdh > b2m > hprt. The results indicated that ef1 alpha can be used as the most stable RG for the tissue distribution analysis of gene expression in H. bleekeri, and ef1 alpha, beta-actin and beta-tubulin was suitable for spleen, head kidney and liver cells, respectively, under LPS and Ploy (I: C) stimulation.
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