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Development of 45 SNP markers in Triplophysa hsutschouensis based on RAD sequencing

文献类型: 外文期刊

作者: Liu, Ya 1 ; Lai, Jiansheng 1 ; Zhao, Zhongmeng 1 ; Ke, Hongyu 1 ; Zhao, Han 1 ; Chen, Yeyu 1 ; Wu, Xiaoyun 1 ; Li, Qiang 1 ;

作者机构: 1.Sichuan Acad Agr Sci, Fishery Inst, Chengdu 611730, Peoples R China

关键词: Triplophysa hsutschouensis; SNP marker; Genetic diversity analysis; RAD-seq

期刊名称:CONSERVATION GENETICS RESOURCES ( 影响因子:0.991; 五年影响因子:0.897 )

ISSN: 1877-7252

年卷期: 2022 年 14 卷 4 期

页码:

收录情况: SCI

摘要: Triplophysa hsutschouensis is an endemic species in China, that is mainly distributed in the Hexi Corridor of Gansu Province. Studies on molecular markers in this species are limited. 45 single nucleotide polymorphism markers were developed and characterized in T. hsutschouensis for the first time using restriction site-associated DNA tag sequencing. The minor allele frequency ranged from 0.033 to 0.500, and the observed and expected heterozygosities ranged from 0.067 to 0.600 and 0.066 to 0.508, respectively. The polymorphism information content ranged from 0.062 to 0.375. Among these SNPs, two loci showed significant departures from Hardy-Weinberg equilibrium after Bonferroni correction. The novel polymorphic SNPs will be helpful for further population genetic analysis and natural resource conservation.

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