OsMADS1 Regulates Grain Quality, Gene Expressions, and Regulatory Networks of Starch and Storage Protein Metabolisms in Rice
文献类型： 外文期刊
作者： Liu, Zhijian 1 ; Li, Penghui 1 ; Yu, Lan 1 ; Hu, Yongzhi 1 ; Du, Anping 1 ; Fu, Xingyue 1 ; Wu, Cuili 1 ; Luo, Dagang 4 ; Hu, Binhua 5 ; Dong, Hui 6 ; Jiang, Haibo 1 ; Ma, Xinrong 1 ; Huang, Weizao 1 ; Yang, Xiaocheng 3 ; Tu, Shengbin 1 ; Li, Hui 1 ;
作者机构： 1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chengdu Univ Technol, Coll Ecol & Environm, Chengdu 610059, Peoples R China
4.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China
5.Sichuan Acad Agr Sci, Biotechnol & Nucl Technol Res Inst, Chengdu 610066, Peoples R China
6.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
关键词： OsMADS1; grain quality; starch metabolism; seed storage protein metabolism; rice
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 9 期
页码：
收录情况： SCI
摘要： OsMADS1 plays a vital role in regulating floret development and grain shape, but whether it regulates rice grain quality still remains largely unknown. Therefore, we used comprehensive molecular genetics, plant biotechnology, and functional omics approaches, including phenotyping, mapping-by-sequencing, target gene seed-specific RNAi, transgenic experiments, and transcriptomic profiling to answer this biological and molecular question. Here, we report the characterization of the `Oat-like rice' mutant, with poor grain quality, including chalky endosperms, abnormal morphology and loose arrangement of starch granules, and lower starch content but higher protein content in grains. The poor grain quality of Oat-like rice was found to be caused by the mutated OsMADS1(Olr) allele through mapping-by-sequencing analysis and transgenic experiments. OsMADS1 protein is highly expressed in florets and developing seeds. Both OsMADS1-eGFP and OsMADS1Olr-eGFP fusion proteins are localized in the nucleus. Moreover, seed-specific RNAi of OsMADS1 also caused decreased grain quality in transgenic lines, such as the Oat-like rice. Further transcriptomic profiling between Oat-like rice and Nipponbare grains revealed that OsMADS1 regulates gene expressions and regulatory networks of starch and storage protein metabolisms in rice grains, hereafter regulating rice quality. In conclusion, our results not only reveal the crucial role and preliminary mechanism of OsMADS1 in regulating rice grain quality but also highlight the application potentials of OsMADS1 and the target gene seed-specific RNAi system in improving rice grain quality by molecular breeding.
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