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OsHARBI1-1 enhances cadmium tolerance in yeast through YAP1 mediated modulation of cell wall integrity genes and catalase genes

文献类型： 外文期刊

作者： Shi, Yang 1 ; Jiang, Nan 1 ; Jiang, Wenjun 1 ; Chen, Ji 3 ; Wu, Lijuan 3 ; Jiao, Yuan 1 ; Zhou, Yingxu 1 ; Wu, Longying 1 ; Huang, Yanyan 4 ; Li, Deqiang 4 ; Kumar, Vinod 5 ; Hu, Binhua 6 ; Huang, Jin 1 ;

作者机构： 1.Chengdu Univ Technol, Coll Ecol & Environm, Chengdu 610059, Peoples R China

2.Sichuan Agr Univ, Coll Resources, Chengdu, Peoples R China

3.Sichuan Agr Univ, Coll Agron, Chengdu, Peoples R China

4.Sichuan Agr Univ, State Key Lab Crop Gene Explorat & Utilizat Southw, Chengdu, Peoples R China

5.Graphic Era Deemed Univ, Dept Food Sci & Technol, Algal Res & Bioenergy Lab, Dehra Dun, India

6.Sichuan Acad Agr Sci, Nucl Technol, Chengdu, Peoples R China

7.Minist Ecol & Environm, Key Lab Monitoring Heavy Met Pollutants, Changsha, Peoples R China

关键词： antioxidant; cadmium tolerance; cell wall; HARBI1; Saccharomyces cerevisiae; YAP1

期刊名称：FASEB JOURNAL （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0892-6638

年卷期： 2025 年 39 卷 3 期

页码：

收录情况： SCI

摘要： Harbinger transposase-derived 1 proteins (HARBI1s) play important roles in plant growth, development, and response to abiotic stress. OsHARBI1-1 has been identified as a gene encoding HARBI1-1 protein in rice and has been shown to be responsive to Cadmium (Cd) stress. However, the function of OsHARBI1-1 protein under heavy metal stress remains unclear. In this study, the function of a novel rice Cd-responsive gene, OsHARBI1-1, under Cd stress was characterized by heterologous expression in yeast. The heterologous expression OsHARBI1-1 conferred yeast with increased tolerance to Cd. In addition, the yeast cells expressing OsHARBI1-1 exhibited enhanced tolerance to Congo red and exhibited an increase in cell wall thickness under Cd stress, suggesting a potential correlation between increased Cd tolerance and cell wall thickness in the transgenic yeast. When OsHARBI1-1 was expressed in triangle yap1 or triangle yap1 triangle ycf1 yeast mutants, there was no significant difference in the tolerance of transgenic yeast to Cd and Congo red, as well as in cell wall thickness compared to the control. Meanwhile, the expression of cell wall integrity (CWI) genes and catalase genes in transgenic yeast was up-regulated in a YAP1-dependent manner under Cd or Congo red stress. The above facts supported the inference that OsHARBI1-1 may counteract Cd toxicity by enhancing the expression of YAP1, thereby increasing the thickness of the cell wall and activating the expression of catalase genes.

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