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Full-length transcriptome sequencing and identification of immune-related genes in the critically endangered Hucho bleekeri

文献类型： 外文期刊

作者： Chen, Yeyu 1 ; Yang, Huanchao 1 ; Chen, Yanling 1 ; Song, Mingjiang 1 ; Liu, Bo 2 ; Song, Jingguo 3 ; Liu, Xin 3 ; Li, Hu 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Fishery Inst, Chengdu 611730, Peoples R China

2.Yaan Fishery Dev Ctr, Yaan 625000, Peoples R China

3.Sichuan Zumuzu River Basin Hydropower Dev Co Ltd, Chengdu 610094, Peoples R China

关键词： Hucho bleekeri; Isoform sequencing; Illumina sequencing; Immune-related genes

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2021 年 116 卷

页码：

收录情况： SCI

摘要： Hucho bleekeri is a glacial relict and critically endangered fish restricted to the Yangtze River drainage in China. The lack of basic genomic information and immune characteristics will hinder the way toward protecting this species. In the present study, we conducted the first transcriptome analysis of H. bleekeri using the combination of SMRT and Illumina sequencing technology. Transcriptome sequencing generated a total of 93,330 nonredundant full-length unigenes with a mean length of 3072 bp. A total of 92,472 (99.08%) unigenes were annotated in at least one of the Nr protein, Swiss-Prot, KEGG, KOG, GO, Nt and Pfam databases. KEGG analysis showed that a total of 7240 unigenes belonging to 28 immune pathways were annotated to the immune system category. Meanwhile, differentially expressed genes between mucosa-associated tissues (skin, gill and hindgut) and systemic-immune tissues (spleen, head kidney and liver) were obtained. Importantly, genes participating in diverse immune signalling pathways and their expression profiles in H. bleekeri were discussed. In addition, a large number of long non-coding RNAs (lncRNAs) and simple sequence repeats (SSRs) were obtained in the H. bleekeri transcriptome. The present study will provide basic genomic information for H. bleekeri and for further research on analysing the characteristics of both the innate and adaptive immune systems of this critically endangered species.

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