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Identification of SNPs and development of allelic specific PCR markers for high molecular weight glutenin subunit D(t)x1.5 from Aegilops tauschii through sequence characterization

文献类型： 外文期刊

作者： Lu, CM 1 ; Yang, WY 1 ; Zhang, WJ 1 ; Lu, BR 1 ;

作者机构： 1.Fudan Univ, Minist Educ, Key Lab Biodivers Sci & Ecol Engn, Inst Biodivers Sci, Shanghai 200433, Peoples R China; Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China

关键词： Aegilops tauschii;Triticum aestivum;synthetic hexaploid wheat;HMW glutenin subunit D(t)1.5;molecular marker;SDS-PAGE;SNP

期刊名称：JOURNAL OF CEREAL SCIENCE （ 影响因子：3.616； 五年影响因子：3.891 ）

ISSN： 0733-5210

年卷期： 2005 年 41 卷 1 期

页码：

收录情况： SCI

摘要： The high molecular weight (HMW) glutenin subunits D(t)x1.5+D(t)y10 from Aegilops tauschii are a novel pair of subunits not detected previously in common wheat (Triticum aestivum). The partial DNA sequences of the x-type HMW glutenin alleles from A. tauschii and synthetic hexaploid wheat samples with different HMW glutenin subunits were charcterised. Five samples were found to contain the HMW glutenin subunit D(t)x1.5 that may affect bread-making quality. Polymorphisms of the DNA sequences were detected among alleles encoding different HMW glutenin subunits and within an allele encoding the same HMW glutenin subunit, such as the D(t)x1.5 subunit. Three single nucleotide polymorphisms (SNPs) that can distinguish the Dx(t)1.5 from D(t)x2, D(t)x5, Dx2 and Dx5 alleles were identified. Allelic specific (AS)-PCR primers were developed based the SNPs located at the non-repetitive N-terminal of the HMW glutenin subunits. The AS-PCR primers can accurately identify accessions containing the D(t)x1.5 subunit from those containing other studied subunits by PCR analysis, suggesting the usefulness of AS-PCR for identifying different HMW glutenin subunits of A. tauschii and synthetic hexaploid wheat. The AS-PCR primers developed based on SNPs in this study are valuable in wheat breeding for effective selection of special HMW glutenin subunits. (C) 2004 Elsevier Ltd. All rights reserved.

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