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Differentiation of the high molecular weight glutenin subunit D-t x2.1 of Aegilops tauschii indicated by partial sequences of its encoding gene and SSR markers

文献类型： 外文期刊

作者： Lu, CM 1 ; Yang, WY 1 ; Lu, BR 1 ;

作者机构： 1.Fudan Univ, Inst Biodivers Sci, Minist Educ State Key Lab Biodivers Sci & Ecol En, Shanghai 200433, Peoples R China; Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China

关键词： Aegilops tauschii;electrophoresis;gene differentiation;HMW-GS;SNP;storage protein

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2005 年 141 卷 1-2 期

页码：

收录情况： SCI

摘要： The high molecular weight glutenin subunits (HMW-GS) are important components of storage proteins in common wheat (Triticum aestivum L.). Conventional techniques for identifying the HMW-GS apply SDS-PAGE. To study differentiation of the HMW glutenin genes, partial DNA sequences of the concerned alleles were characterized from eight accessions of hexaploid synthetic wheat and common wheat varieties, and compared with the available sequences. SSR markers based on, and generated from, DNA sequences of the D genome of common wheat and Aegilops tauschii were analyzed to estimate genetic relationships of the accessions. Results showed that the HMW-GS D(t)x2.1 determined by SDS-PAGE could be identified as two types at the DNA sequence level. One type, designated as D(t)x2.1a, showed identical SNPs to the D(t)x1.5 allele and another type, designated as D(t)x2.1b, had different characteristics. The SSR analysis revealed a relatively close genetic relationship of the two accessions containing the D(t)x2.1a allele to the accessions containing the D(t)x1.5 allele. It was concluded that the conventional SDS-PAGE has limitations in determining some HMW-GS, because the minor changes on the DNA sequence of a particular glutenin subunit cannot be detected at protein level, and that the AS-PCR method based on variation of DNA sequences of particular genes will provide a more powerful tool for identifying the HMW-GS. The differentiation of the genes encoding the HMW-GS is probably more rapid than expected. The close relationship between evolutionary changes of the microsatellite DNA and the HMW-GS genes detected in this study needs to be further investigated.

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