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Molecular characterization and validation of adult-plant stripe rust resistance gene Yr86 in Chinese wheat cultivar Zhongmai 895

文献类型： 外文期刊

作者： Zhu, Zhanwang 1 ; Cao, Qiang 1 ; Han, Dejun 3 ; Wu, Jianhui 3 ; Wu, Ling 4 ; Tong, Jingyang 1 ; Xu, Xiaowan 1 ; Yan, Jun 5 ; Zhang, Yong 1 ; Xu, Kaijie 5 ; Wang, Fengju 1 ; Dong, Yachao 1 ; Gao, Chunbao 2 ; He, Zhonghu 1 ; Xia, Xianchun 1 ; Hao, Yuanfeng 1 ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Inst Crop Sci, Beijing 100081, Peoples R China

2.Hubei Acad Agr Sci, Food Crops Inst, Wheat Dis Biol Res Stn Cent China, Wuhan 430064, Hubei, Peoples R China

3.Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China

4.Sichuan Acad Agr Sci, Crop Res Inst, Environm Friendly Crop Germplasm Innovat & Genet I, Chengdu 610066, Sichuan, Peoples R China

5.CAAS, Inst Cotton Res, Anyang 455000, Henan, Peoples R China

6.CAAS, CIMMYT China Off, Beijing 100081, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2023 年 136 卷 6 期

页码：

收录情况： SCI

摘要： Key messageAdult-plant stripe rust resistance gene Yr86 in Chinese wheat cultivar Zhongmai 895 was mapped to the physical interval 710.2-713.2 Mb on the long arm of chromosome 2A.Adult-plant resistance to stripe rust is generally more durable than all-stage resistance. Chinese wheat cultivar Zhongmai 895 showed stable stripe rust resistance at the adult-plant stage. To map the genetic loci underlying its resistance, 171 doubled haploid (DH) lines from a Yangmai 16/Zhongmai 895 cross were genotyped with the wheat 660 K SNP chip. Disease severities of the DH population and parents were assessed in four environments. A major QTL designated QYryz.caas-2AL was mapped to interval 703.7-715.3 Mb on the long arm of chromosome 2A using both chip-based and KASP (kompetitive allele-specific PCR) marker-based methods, explaining 31.5 to 54.1% of the phenotypic variances. The QTL was further validated in an F-2 population of cross Emai 580/Zhongmai 895 with 459 plants and a panel of 240 wheat cultivars using KASP markers. Three reliable KASP markers predicted a low frequency (7.2-10.5%) of QYryz.caas-2AL in the test panel and remapped the gene to the physical interval 710.2-713.2 Mb. Based on different physical positions or genetic effects from known genes or QTL on chromosome arm 2AL, the gene was predicted to be a new one for adult-plant stripe rust resistance and was named Yr86. Twenty KASP markers linked to Yr86 were developed in this study based on wheat 660 K SNP array and genome re-sequencing. Three of them are significantly associated with stripe rust resistance in natural population. These markers should be useful for marker-assisted selection and also provide a starting point for fine mapping and map-based cloning of the new resistance gene.

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