文献类型： 外文期刊

作者： Shen, Jinjuan ¹ ; Cai, Pingzhong ¹ ; Qing, Feng ¹ ; Zhang, Zhiyong ¹ ; Wang, Guixue ¹ ;

作者机构： 1.Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Lab Mechanodev Biol,Bioengn Coll, Chongqing 400044, Peoples R China

2.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol, Chengdu 610066, Peoples R China

期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.972； 五年影响因子：0.988 ）

ISSN： 0556-3321

年卷期： 2012 年 44 卷 2 期

页码：

收录情况： SCI

摘要： Based on the expression vector pBI121, we successfully constructed a plant overexpression vector of Hspa4 gene fusing with selective maker gene (hygromycin-resistance gene) driven by the Ubi-1 promoter (pBI121-Ubi-Hpt-Hspa4, p121UHH). The plant expression vectors p121UHH and pCAMBIA1301-Ubi-Hspa4 (p1301UH) were transformed into the rice callus, mediated by Agrobacterium tumefaciens. We screened 17 p121 UHH-positive transgenic plants and 15 p1301UH-positive transgenic plants by the hygromycin-resistance gene. The pick-up rate of the resistance callus was 51.7% and 42.5%, respectively, and the rate of regeneration for the resistance callus was 51.2% and 49.1%, respectively. The result of polymerase chain reaction (PCR) identification indicated that the pick-up rate of positive transgenic plants was 51.7% and 42.5% and the total transformation efficiency was 16.5% and 6.2%, and the former was 2.66 times of the later. The results of the experiment indicate that the possibility of the appearance of false positive results in the fusing of a plant overexpression vector with a selective maker gene is much less.