CONSTRUCTION OF EUKARYOTIC PLANT EXPRESSION VECTOR WITH THE SAG12 PROMOTER AND THE LEAF SENESCENCE-RELATED IPT GENE AND ITS GENETIC TRANSFORMATION IN RICE
文献类型： 外文期刊
作者： Cai, Pingzhong 2 ; Qian, Qiufang 1 ; Kang, Zhenhui 1 ; Zhang, Zhiyong 2 ; Wang, Guixue 1 ;
作者机构： 1.Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Lab Mechanodev Biol,Bioengn Coll, Chongqing 400044, Peoples R China
2.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol, Chengdu 610066, Peoples R China
期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.972； 五年影响因子：0.988 ）
ISSN： 0556-3321
年卷期： 2012 年 44 卷 6 期
页码：
收录情况： SCI
摘要： A pair of primers was designed according to the sequences published by GenBank to amplify the isopentenyl transferase gene (ipt gene) of the plasmid in Agrobacterium tumefaciens (strain C58). The 5'-untranslated sequence of the senescence-associated SAG12 gene of Arabidopsis was isolated by the polymerase chain reaction (PCR). In addition, we constructed the plant-expression vectors (pBI121-ipt and pCAMBIA1301-SAG12-ipt), which were regulated by the SAG12 promoter and the ipt gene. The plant-expression vector was used to transform the embryos of rice cultivar Zhonghua 16 with the help of the A. tumefaciens system. Plantlets were regenerated in vitro by selection on medium containing hygromycin. PCR amplification showed that the target gene was successfully integrated into the transformed plants.
- 相关文献
作者其他论文 更多>>
-
Metabolomics analysis of five cultivars of Sorghum bicolor reveals the contribution of flavonoid pathway to tannin content
作者：Ni, Xianlin;Long, Wenjing;Ni, Xianlin;Gong, Lijuan;Kang, Zhenhui;Zheng, Jia;Li, Yanghua
关键词：
-
Telomere-to-telomere genome assemblies of three silkworm strains with long-term pupal characteristics
作者：Wan, Linrong;Liu, Binbin;Su, Siyuan;Zhang, Zhiyong;Jiang, Yaming;Zou, Bangxing;Liu, Junfeng;Du, Zhouhe;Zhang, Youhong;Xiao, Wenfu;Wan, Linrong;Chen, Peng;Deng, Cao;Deng, Cao
关键词：
-
Cu/Zn Superoxide Dismutase and Catalase of Yangtze Sturgeon, Acipenser dabryanus: Molecular Cloning, Tissue Distribution and Response to Fasting and Refeeding
作者：Shi, Qingchao;Xiong, Xiaoqin;Wen, Zhengyong;Qin, Chuanjie;Li, Rui;Zhang, Zhiyong;Gong, Quan;Wu, Xiaoyun
关键词：Yangtze sturgeon; Cu; Zn-SOD; CAT; fasting; refeeding
-
A PRIMARY STUDY OF HIGH PERFORMANCE TRANSGENIC RICE THROUGH MAIZE UBI-1 PROMOTER FUSING SELECTIVE MAKER GENE
作者：Cai, Pingzhong;Qing, Feng;Wang, Guixue;Cai, Pingzhong
关键词：
-
The Maize Imprinted Gene Floury3 Encodes a PLATZ Protein Required for tRNA and 5S rRNA Transcription through Interaction with RNA Polymerase III
作者：Wang, Jiechen;Ye, Jianwei;Zheng, Xixi;Fu, Miaomiao;Wang, Qiong;Zhang, Zhiyong;Wu, Yongrui;Li, Qi;Zheng, Xixi;Fu, Miaomiao;Xiang, Xiaoli;Li, Changsheng
关键词：
-
OsEXPB2, a beta-expansin gene, is involved in rice root system architecture
作者：Wenwen, Yihao;Zang, Guangchao;Kang, Zhenhui;Huang, Junli;Wang, Guixue;Zhang, Zhiyong
关键词：Expansins;OsEXPB2;Root system architecture;Plant development
