文献类型： 外文期刊

作者： Zhou, J. P. ¹ ; Yao, C. H. ¹ ; Yang, E. N. ² ; Yin, M. Q. ¹ ; Liu, C. ¹ ; Ren, Z. L. ¹ ;

作者机构： 1.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Sichuan, Peoples R China

2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu, Sichuan, Peoples R China

3.Sichuan Agr Univ, State Key Lab Plant Breeding & Genet, Chengdu, Sichuan, Peoples R China

关键词： Aegilops biuncialis;Bread-making quality;C-banding;High molecular weight glutenin

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2014 年 13 卷 1 期

页码：

收录情况： SCI

摘要： In this study, a new disomic addition line, 12-5-2, with 44 chromosomes that was derived from BC3F2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. 12-5-2 was immune to both powdery mildew and stripe rust and has stable fertility. Fluorescence in situ hybridization and C-banding revealed that 12-5-2 was a 1U(b) disomic addition line (ADL1U(b)). The seed storage protein electrophoresis showed that 12-5-2 presented all high molecular weight glutenin subunits (7 + 8 and 2 + 12) of CN19 and 2 new subunits that were designated Ux and Uy. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness of 12-5-2 were significantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1U(b)-specific polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1041, TNAC1071, TNAC1-01, and TNAC1-04, were also obtained. The new ADL1U(b) 12-5-2 could be a valuable source for wheat improvement, especially for wheat end-product quality and resistance to disease.