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Characterization of a New Wheat-Aegilops biuncialis 1M(b)(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit

文献类型： 外文期刊

作者： Zhou, J. P. 1 ; Cheng, Y. 1 ; Zang, L. L. 1 ; Yang, E. N. 2 ; Liu, C. 3 ; Zheng, X. L. 1 ; Deng, K. J. 1 ; Zhu, Y. Q. 4 ; Zh 1 ;

作者机构： 1.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Peoples R China

2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China

3.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Peoples R China

4.Sichuan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Chengdu 6110066, Peoples R China

关键词： wheat;Aegilops biuncialis;bread-making quality;HMW-GS;multi-colour FISH

期刊名称：CEREAL RESEARCH COMMUNICATIONS （ 影响因子：0.85； 五年影响因子：1.067 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： In this study, a new substitution line, 12-5-1, with 42 chromosomes that was derived from BC3F2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. The 12-5-1 was immune to both powdery mildew and stripe rust and has stable fertility. Multi-color fluorescence in situ hybridization indicated that 12-5-1 was a substitution line 1M(b)(1B). The seed storage protein electrophoresis showed that 12-5-1 presented high molecular weight glutenin subunits (2 + 12) of CN19 and a new subunit designated as M which apparently originated from parent Ae. biuncialis, and absent 7 + 8 subunits. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness and mixing time of 12-5-1 were signifiantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1M(b)-specifi polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1026, TNAC1041, TNAC1-02 and TNAC1-04, were also obtained. The new substitution line 1M(b)(1B) 12-5-1 could be a valuable source for wheat improvement, especially for wheat end product quality and resistance to disease.

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