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100 Gy Co-60 gamma-Ray Induced Novel Mutations in Tetraploid Wheat

文献类型: 外文期刊

作者: Yang, Chuntao 2 ; Zhu, Jianshu 1 ; Jiang, Yun; Wang, Xiaolu 1 ; Gu, Mengxue 1 ; Wang, Yi 1 ; Kang, Houyang 1 ; Fan, Xi 1 ;

作者机构: 1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China

2.Sichuan Agr Univ, Minist Educ, Key Lab Crop Genet Resources & Improvement, Chengdu 611130, Sichuan, Peoples R China

3.Sichuan Agr Univ, Minist Educ, Key Lab Crop Genet Resources & Improvement, Chengdu 611130, Sichuan, Peoples

期刊名称:SCIENTIFIC WORLD JOURNAL ( 影响因子:1.219; 五年影响因子:1.3 )

ISSN: 1537-744X

年卷期: 2014 年

页码:

收录情况: SCI

摘要: 10 accessions of tetraploid wheat were radiated with 100 Gy Co-60 gamma-ray. The germination energy, germination rate, special characters (secondary tillering, stalk with wax powder, and dwarf), meiotic process, and high-molecular-weight glutenin subunits (HMW-GSs) were observed. Different species has different radiation sensibility. With 1 seed germinated (5%), T. dicoccum(PI434999) is the most sensitive to this dose of radiation. With a seed germination rate of 35% and 40%, this dose also affected T. polonicum (As304) and T. carthlicum (As293). Two mutant dwarf plants, T. turgidum (As2255) 253-10 and T. polonicum (As302) 224-14, were detected. Abnormal chromosome pairings were observed in pollen mother cells of both T. dicoccoides (As835) 237-9 and T. dicoccoides (As838) 239-8 with HMW-GS 1Ax silent in seeds from them. Compared with the unirradiated seed of T. polonicum (As304) CK, a novel HMW-GS was detected in seed of T. polonicum (As304) 230-7 and its electrophoretic mobility was between 1By8 and 1Dy12 which were the HMW-GSs of Chinese Spring. These mutant materials would be resources for wheat breeding.

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