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Canolol as a key equivalent for phenolic content quantification in rapeseed oil via Folin-Ciocalteu method

文献类型： 外文期刊

作者： Hu, Zihui 1 ; Xiong, Wei 2 ; Liang, Qiang 2 ; Wang, Chenzhi 2 ; Xuan, Pu 2 ; Li, Yan 2 ; Hua, Xin 2 ; Guo, Hongxiang 3 ; Yao, Yingzheng 1 ;

作者机构： 1.Chengdu Univ, Coll Food & Biol Engn, Chengdu 610106, Peoples R China

2.Sichuan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Chengdu 610066, Peoples R China

3.Chengdu Newshine Oil & Grain Co Ltd, Chengdu 611533, Peoples R China

关键词： Rapeseed oil; Phenols; 4-Vinylsyringol (canolol); Ultra-high-performance liquid chromatography (UHPLC); Folin-Ciocalteu (F-C) method

期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：3.2； 五年影响因子：3.7 ）

ISSN： 1438-2377

年卷期： 2025 年 251 卷 6 期

页码：

收录情况： SCI

摘要： Total phenolic contents (TPC) of 10 simulated extract solutions and 24 rapeseed oil samples were quantified by Folin-Ciocalteu (F-C) method with canolol, sinapic acid, and gallic acid as equivalent chemicals, respectively. Solid-phase extraction (SPE) and liquid-liquid extraction (LLE) were used for total phenolic extracting. Results revealed that LLE extracting on average 23.71% more total phenols than SPE. For simulated extract solutions 1-7, containing higher proportions of canolol, canolol was a superior equivalent, yielding TPC that were consistently closer to those obtained via ultra-high-performance liquid chromatography (UHPLC); while for simulated extract solution 8-10, which contained uniformly distributed phenolic compounds, sinapic acid provided more accurate quantification. Furthermore, for rapeseed oil samples (n = 23) with > 60% canolol, TPCs calculated by canolol using the F-C method were closer to the UHPLC results (p > 0.05). However, for the sample with < 40% canolol, TPC calculated by sinapic acid using the F-C method was closer to the UHPLC result (p > 0.05). This indicates that canolol is the dominant phenolic compound in most rapeseed oil. Therefore, the F-C method can be used as an alternative to UHPLC for TPC quantification, using canolol as the equivalent chemical. In addition, when chemical methods (e.g. F-C method) are used for determination of a class of substance (e.g. TPC), it is necessary to identify the dominant components and using it as an equivalent chemical, which may help to get a more accurate result.

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