文献类型： 外文期刊

作者： Sun, Mengjing ¹ ; Luo, Qiaoling ² ; Zheng, Qi ² ; Tong, Jingyang ¹ ; Wang, Yue ¹ ; Song, Jie ¹ ; Zhang, Yelun ³ ; Pu, Zongjun ⁴ ; Zheng, Jianmin ⁴ ; Liu, Lianzheng ⁵ ; Zhou, Anding ⁵ ; Rasheed, Awais ¹ ; Li, Ming ¹ ; Cao, Shuanghe ¹ ; Xia, Xianchun ¹ ; He, Zhonghu ¹ ; Hao, Yuanfeng ¹ ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Inst Crop Sci, 12 Zhongguancun South St, Beijing 100081, Peoples R China

2.Chinese Acad Sci, Inst Genet & Dev Biol, Innovat Acad Seed Design, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

3.Inst Cereal & Oil Crops, Hebei Acad Agr & Forestry Sci, Hebei Lab Crop Genet & Breeding, Shijiazhuang 050031, Hebei, Peoples R China

4.Sichuan Acad Agr Sci, Inst Crop Sci, Chengdu 610066, Sichuan, Peoples R China

5.Xinjiang Acad Agr Sci, Inst Grain Crops, Urumqi 830000, Xinjiang, Peoples R China

6.Int Maize & Wheat Improvement Ctr CIMMYT China Off, C-O CAAS,12 Zhongguancun South St, Beijing 100081, Peoples R China

7.Quaid i Azam Univ, Dept Plant Sci, Islamabad 45320, Pakistan

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2023 年 136 卷 10 期

页码：

收录情况： SCI

摘要： Key messageMajor QTL for grain zinc and iron concentrations were identified on the long arm of chromosomes 2D and 6D. Gene-based KASP markers were developed for putative candidate genes TaIPK1-2D and TaNAS10-6D.AbstractMicronutrient malnutrition is one of the most common public health problems in the world. Biofortification, the most attractive and sustainable solution to surmount malnutrition requires the development of micronutrient enriched new crop cultivars. In this study, two recombinant inbred line (RIL) populations, ZM175/XY60 and ZM175/LX987, were used to identify QTL for grain zinc concentration (GZnC), grain iron concentration (GFeC) and thousand grain weight (TGW). Eight QTL for GZnC, six QTL for GFeC and five QTL for TGW were detected. Three QTL on chromosomes 2DL and 4BS and chromosome 6A showed pleiotropic effects on all three traits. The 4BS and 6A QTL also increased plant height and might be Rht-B1a and Rht25a, respectively. The 2DL locus within a suppressed recombination region was identified in both RIL populations and the favorable allele simultaneously increasing GZnC, GFeC and TGW was contributed by XY60 and LX987. A QTL on chromosome 6DL associated only with GZnC was detected in ZM175/XY60 and was validated in JD8/AK58 RILs using kompetitive allele-specific PCR (KASP) marker K_AX-110119937. Both the 2DL and 6DL QTL were new loci for GZnC. Based on gene annotations, sequence variations and expression profiles, the phytic acid biosynthesis gene TaIPK1-2D and nicotianamine synthase gene TaNAS10-6D were predicted as candidate genes. Their gene-based KASP markers were developed and validated in a cultivar panel of 343 wheat accessions. This study investigated the genetic basis of GZnC and GFeC and provided valuable candidate genes and markers for breeding Zn- and Fe-enriched wheat.