Gliotoxin Is an Important Secondary Metabolite Involved in Suppression of Sclerotium rolfsii of Trichoderma virens T23
文献类型： 外文期刊
作者： Hua, Lixia 1 ; Zeng, Hualan 1 ; He, Lian 1 ; Jiang, Qiuping 1 ; Ye, Pengsheng 1 ; Liu, Yong 1 ; Sun, Xiaofang 1 ; Zhang, Min 1 ;
作者机构： 1.Sichuan Acad Agr Sci, Ind Crop Res Inst, Chengdu 610300, Peoples R China
2.MOA Key Lab Integrated Management Pests Crops Sou, Chengdu 610066, Peoples R China
关键词： biocontrol agent; biological control; gli gene cluster; gliotoxin; Sclerotium rolfsii; Trichoderma spp.
期刊名称：PHYTOPATHOLOGY （ 影响因子：4.01； 五年影响因子：4.457 ）
ISSN： 0031-949X
年卷期： 2021 年 111 卷 10 期
页码：
收录情况： SCI
摘要： Sclerotium rolfsii causes destructive soilborne disease in numerous plant species, and biological control may be a promising and sustainable approach for suppressing this widespread pathogen. In this study, the antagonistic effect against S. rolfsii of 10 Trichoderma strains was tested by the dual culture method, and a gliotoxin-producing strain, T. virens T23, was shown to be the most effective, inhibiting growth of S. rolfsii in vitro by 70.2%. To clarify the antagonistic mechanism and gliotoxin biosynthesis regulation of T23, a gliotoxin-deficient mutant was constructed via Agrobacterium tumefaciens-mediated gene knockout in vivo. As expected, disruption of the gene located in the putative gliotoxin biosynthesis gene cluster, gliI-T, resulted in gliotoxin deficiency and attenuation of the antagonistic effect against S. rolfsii, indicating that gliotoxin biosynthesis is regulated by gliI-T and that gliotoxin is an important antifungal metabolite of T23. Transmission electron microscopy revealed that gliotoxin treatment caused marked alterations of the hyphal cells of S. rolfsii depending on the drug concentration, whereby one of the prominent structural alterations was a reduction in the number and length of mitochondrial cristae. When S. rolfsii was exposed to 30 lg/ml of gliotoxin for 12 h, striking plasmolysis and ultrastructural changes were induced. The results demonstrated that gliotoxin is an important secondary metabolite of T. virens T23 in its antagonism against S. rolfsii.
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