文献类型： 外文期刊

作者： Li, Yinghui ¹ ; Wei, Zhen-Zhen ¹ ; Sela, Hanan ¹ ; Govta, Liubov ¹ ; Klymiuk, Valentyna ¹ ; Roychowdhury, Rajib ¹ ; Chawla, Harmeet Singh ⁴ ; Ens, Jennifer ⁴ ; Wiebe, Krystalee ⁴ ; Bocharova, Valeria ¹ ; Ben-David, Roi ¹ ; Pawar, Prerna B. ¹ ; Zhang, Yuqi ⁷ ; Jaiwar, Samidha ¹ ; Molnar, Istvan ⁸ ; Dolezel, Jaroslav ⁸ ; Coaker, Gitta ⁹ ; Pozniak, Curtis J. ⁴ ; Fahima, Tzion ¹ ;

作者机构： 1.Univ Haifa, Inst Evolut, IL-3498838 Haifa, Israel

2.Univ Haifa, Dept Evolutionary & Environm Biol, IL-3498838 Haifa, Israel

3.Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu 610066, Peoples R China

4.Univ Saskatchewan, Crop Dev Ctr, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada

5.Univ Saskatchewan, Dept Plant Sci, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada

6.Agr Res Org ARO, Dept Vegetables, Inst Plant Sci, Volcani Ctr, IL-7505101 Rishon Leziyyon, Israel

7.China Agr Univ, Dept Crop Genom & Bioinformat, Beijing 100094, Peoples R China

8.Czech Acad Sci, Ctr Reg Hana Biotechnol & Agr Res, Inst Expt Bot, Slechtitel U 31, Olomouc 77900, Czech Republic

9.Univ Calif Davis, Plant Pathol Dept, Davis, CA 95616 USA

10.Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada

11.ELKH, Ctr Agr Res, Agr Inst, Martonvasar, Hungary

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2024 年 5 卷 1 期

页码：

收录情况： SCI

摘要： Gene cloning in repeat-rich polyploid genomes remains challenging. Here, we describe a strategy for overcoming major bottlenecks in cloning of the powdery mildew resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat. A conventional positional cloning approach was not effective owing to suppressed recombination. Chromosome sorting was compromised by insufficient purity. A Pm69 physical map, constructed by assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed a rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster with structural variations. A single candidate NLR was identified by anchoring RNA sequencing reads from susceptible mutants to ONT contigs and was validated by virus-induced gene silencing. Pm69 is likely a newly evolved NLR and was discovered in only one location across the wild emmer wheat distribution range in Israel. Pm69 was successfully introgressed into cultivated wheat, and a diagnostic molecular marker was used to accelerate its deployment and pyramiding with other R-genes.