四川省农业科学院机构知识库

Isolation of two new retrotransposon sequences and development of molecular and cytological markers for Dasypyrum villosum (L.)

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文献类型：外文期刊

作者： Zhang, Jie ⁴ ; Jiang, Yun ¹ ; Xuan, Pu ³ ; Guo, Yuanlin ¹ ; Deng, Guangbing ² ; Yu, Maoqun ² ; Long, Hai ² ;

作者机构： 1.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol Res, Chengdu 610061, Sichuan, Peoples R China

2.Chinese Acad Sci, Chengdu Inst Biol, 9 Sect 4,Renmin South Rd, Chengdu 610041, Sichuan, Peoples R China

3.Sichuan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Chengdu 610066, Sichuan, Peoples R China

4.Minist Agr, Key Lab Wheat Biol & Genet Improvement Southweste, Chengdu 610066, Sichuan, Peoples R China

关键词： Dasypyrum villosum;Retrotransposon;SCAR marker;Karyotype;FISH

期刊名称：GENETICA （影响因子：1.082；五年影响因子：1.489 ）

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收录情况： SCI

摘要： Dasypyrum villosum is a valuable genetic resource for wheat improvement. With the aim to efficiently monitor the D. villosum chromatin introduced into common wheat, two novel retrotransposon sequences were isolated by RAPD, and were successfully converted to D. villosum-specific SCAR markers. In addition, we constructed a chromosomal karyotype of D. villosum. Our results revealed that different accessions of D. villosum showed slightly different signal patterns, indicating that distribution of repeats did not diverge significantly among D. villosum accessions. The two SCAR markers and FISH karyotype of D. villosum could be used for efficient and precise identification of D. villosum chromatin in wheat breeding.

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