Quantification of aflatoxin M1 in raw milk by a core-shell column on a conventional HPLC with large volume injection and step gradient elution
文献类型： 外文期刊
作者： Mao, Jianfei 2 ; Lei, Shaorong 1 ; Liu, Yunhua 1 ; Xiao, Dan 3 ; Fu, Chengping 1 ; Zhong, Lingli 1 ; Ouyang, Huaxue 1 ;
作者机构： 1.Sichuan Acad Agr Sci, Anal & Testing Ctr, Chengdu 610066, Peoples R China
2.Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Chengd, Chengdu 610066, Peoples R China
3.Sichuan Univ, Coll Chem, Chengdu 610065, Peoples R China
4.Sichuan Univ, Coll Chem Engn, Chengd
关键词： HPLC-FLD;Aflatoxin M1;lmmunoaffinity column cleanup;Core-shell column;Step gradient elution;Raw milk
期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： The immunoaffinity column cleanup method coupled with HPLC separation and fluorescence detection is still one of the most frequently used quantitative methods for routine analyses of AFM1. In this study, aflatoxin M1 (AFM1) was quantified with a chromatography column packed with 2.6 gm core-shell particles on a conventional HPLC. A large volume of solvent (100 mu L) was injected into the highly efficient column without any noticeable reduction in separation performance with the help of stepwise gradient elution. The instrumental conditions were optimized by response surface analysis methodology (RSM) with a three-level three-factor Box-Behnken design.
- 相关文献
作者其他论文 更多>>
-
Tailoring sodium storage with phosphorus-doped hollow carbon nanospheres: Atomic-level engineering of charge transport and ion dynamics
作者：Wang, Xilong;Zhang, Deng;Zheng, Qiaotian;Song, Jie;Chen, Dawei;Yan, Debing;He, Bin;Zhao, Qian;Tang, Mao;Xiao, Dan;Meng, Yan;Ying, Jing
关键词：Cassava starch; Phytic acid; Hollow nanosphere; Hard carbon; Sodium-ion batteries
-
A Multiplex Real-Time PCR for Qualitative Detection of Mutton, Pork, Chicken, and Duck in Processed Meat Products
作者：Chang, Lijuan;Fu, Chengping;Deng, Mengxue;Li, Yuanhong
关键词：meat adulteration; multiplex real-time PCR; processed meat products; qualitative detection
-
Reliable multiplex real-time PCR method for detecting adulteration in processed beef products
作者：Chang, Lijuan;Fu, Chengping;Huang, Ping;Li, Yuanhong;Liu, Yongling;Lu, Fan
关键词：Multiplex real-time PCR; Meat adulteration; Qualitative detection; Beef
-
Real-time PCR method based on single-copy nuclear DNA sequences for the quantitative detection of pork adulteration in processed beef products
作者：Chang, Lijuan;Huang, Ping;Fu, Chengping;Li, Yuanhong;Liu, Yongling;Long, Ailin;Liu, Jikai;Gao, Yongfeng;Liu, Wenjuan
关键词：Food safety; Real-time polymerase chain reaction; Pork; Beef; Meat adulteration; Quantification method
-
Comparative Analysis of Nutrients, Phytochemicals, and Minerals in Colored Sweet Potato (Ipomoea batatas L.) Roots
作者：Zhao, Shan;Zhong, Lingli;Li, Xi;Qin, Lin;Zhou, Ya;Lei, Xinyu;Zheng, Xingguo;Jin, Keting;Hou, Xue;Song, Jun;Pu, Zhigang;Lang, Tao;Zhang, Cong;Feng, Junyan
关键词：sweet potato roots; flesh colors; nutrients; phytochemicals; minerals
-
Self-replicating catalyzed hairpin assembly for rapid aflatoxin B1 detection
作者：Zhao, Lijun;Zhao, Lijun;Mao, Jianfei;Hu, Li;Yang, Xiaofeng;Zhang, Shu
关键词：
-
A highly sensitive visual sensor for tetracycline in food samples by a double-signal response fluorescent nanohybrid
作者：Hu, Jing;Yang, Xiaofeng;Peng, Qianqian;Wang, Fengyi;Zhu, Yue;Hu, Xuan;Zheng, Baozhan;Du, Juan;Xiao, Dan;Zheng, Baozhan;Du, Juan;Xiao, Dan;Yang, Xiaofeng
关键词：Ratiometric fluorescent probe; Visual detection; Paper-based test strip; Tetracycline; Food samples
