Transcriptomic and metabolomic analyses reveals keys genes and metabolic pathways in tea (Camellia sinensis) against six-spotted spider mite (Eotetranychus Sexmaculatus)
文献类型： 外文期刊
作者： Wang, Xiaoping 1 ; Xiang, Yunjia 2 ; Sun, Minshan 3 ; Xiong, Yuanyuan 1 ; Li, Chunhua 1 ; Zhang, Ting 1 ; Ma, Weiwei 1 ; Wang, Yun 1 ; Liu, Xiao 1 ;
作者机构： 1.Sichuan Acad Agr Sci, Tea Res Inst, Tea Refining & Innovat Key Lab Sichuan Prov, Chengdu, Peoples R China
2.Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu, Peoples R China
3.Henan Assist Res Biotechnol Co Ltd, Zhengzhou, Peoples R China
关键词： Tea plant; Spider mite; Transcriptome; Metabolome; Plant-insect interaction
期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.3； 五年影响因子：5.9 ）
ISSN： 1471-2229
年卷期： 2023 年 23 卷 1 期
页码：
收录情况： SCI
摘要： Background Six-spotted spider mite (Eotetranychus sexmaculatus) is one of the most damaging pests of tea (Camellia sinensis). E. sexmaculatus causes great economic loss and affects tea quality adversely. In response to pests, such as spider mites, tea plants have evolved resistance mechanisms, such as expression of defense-related genes and defense-related metabolites.Results To evaluate the biochemical and molecular mechanisms of resistance in C. sinensis against spider mites, "Tianfu-5" (resistant to E. sexmaculatus) and "Fuding Dabai" (susceptible to E. sexmaculatus) were inoculated with spider mites. Transcriptomics and metabolomics based on RNA-Seq and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) technology were used to analyze changes in gene expression and metabolite content, respectively.RNA-Seq data analysis revealed that 246 to 3,986 differentially expressed genes (DEGs) were identified in multiple compared groups, and these DEGs were significantly enriched in various pathways, such as phenylpropanoid and flavonoid biosynthesis, plant-pathogen interactions, MAPK signaling, and plant hormone signaling. Additionally, the metabolome data detected 2,220 metabolites, with 194 to 260 differentially abundant metabolites (DAMs) identified in multiple compared groups, including phenylalanine, lignin, salicylic acid, and jasmonic acid. The combined analysis of RNA-Seq and metabolomic data indicated that phenylpropanoid and flavonoid biosynthesis, MAPK signaling, and Ca2+-mediated PR-1 signaling pathways may contribute to spider mite resistance.Conclusions Our findings provide insights for identifying insect-induced genes and metabolites and form a basis for studies on mechanisms of host defense against spider mites in C. sinensis. The candidate genes and metabolites identified will be a valuable resource for tea breeding in response to biotic stress.
- 相关文献
作者其他论文 更多>>
-
Multi-Omics analysis reveals the sensory quality and fungal communities of Tibetan teas produced by wet- and dry-piling fermentation
作者：Chen, Shengxiang;Zhang, Mengxue;Luo, Shijie;Ning, Meiyi;Chen, Yuxi;Tan, Liqiang;Liu, Chen;Tang, Xiaobo;Liu, Xiao;Zhang, Ting;Zheng, Liang;Saarloos, Aafke
关键词：Ya'an Tibetan tea; Wet-pile fermentation; Dry-pile fermentation; Differential metabolites; Fungal communities
-
A combined drying process involving hot air and roasting for improving summer congou black tea quality
作者：Liu, Fei;Li, Chunhua;Wang, Yun;Zhang, Juan;Tang, Xiaobo;Zhang, Ting;Zhu, Hongkai;Liu, Yueyun
关键词：Black tea; Drying technology; Flavor quality; Amino acids; Flavonoids; Volatile compounds
-
Ultrasonic modification on the physicochemical, in vitro simulated digestion and colonic fermentation properties of Golden Flower Tibetan Tea Polysaccharides
作者：Xia, Chen;Zhao, Sijia;Yu, Manyou;Deng, Junlin;Xiang, Zhuoya;Chen, Jian;Tang, Xiaobo;Zhu, Yongqing;Zhao, Sijia;Zeng, Ruiqi;Zhao, Jiayuan;Zhang, Ting;Shi, Liugang;Jin, Yan;Wu, Bin
关键词：Golden flower Tibetan tea polysaccharides (GFTPS); Ultrasonic modification; Antioxidant activity; In vitro digestion; Short-chain fatty acids (SCFAs); Prebiotic potential
-
Genome-Wide Identification of the PIP5K Gene Family in Camellia sinensis and Their Roles in Metabolic Regulation
作者：Wang, Xiaoping;Xiong, Yuanyuan;Tang, Xiaobo;Zhang, Ting;Ma, Weiwei;Wang, Yun;Li, Chunhua
关键词：tea; PIP5K; genome-wide; phylogenetic analysis; metabolic regulation; expression pattern
-
Restriction site-associated DNA sequencing (RAD-seq) of tea plant (Camellia sinensis) in Sichuan province, China, provides insights into free amino acid and polyphenol contents of tea
作者：Wang, Xiaoping;Sun, Minshan;Xiong, Yuanyuan;Liu, Xiao;Li, Chunhua;Wang, Yun;Tang, Xiaobo
关键词：
-
Effect of Pleurotus eryngii mycelial fermentation on the composition and antioxidant properties of tartary buckwheat
作者：Li, Lijiao;Huang, Jingwei;Zhang, Ting;Wu, Qian;Xiang, Peng;Zou, Liang;Li, Qiang;Cao, Xiaonian;Shen, Caihong;Cao, Xiaonian;Shen, Caihong;Li, Qiang;Huang, Jingwei;Li, Jun;Li, Qiang
关键词：Solid-state fermentation technology; Extraction process optimization; Active ingredients; Antioxidant activity
-
Phytochemical composition of Tibetan tea fermented by Eurotium cristatum and its effects on type 1 diabetes mice and gut microbiota
作者：Deng, Junlin;Luo, Kebin;Xia, Chen;Zhu, Yongqing;Xiang, Zhuoya;Zhu, Boyu;Chen, Jian;Luo, Kebin;Lyu, Xiaohua;Luo, Kebin;Lyu, Xiaohua;Tang, Xiaobo;Zhang, Ting;Shi, Liugang
关键词：Theabrownin; Tibetan tea extract; Eurotium cristatum fermentation; Diabetes mellitus; Gut microbiota
