文献类型： 外文期刊
作者： Zhang, Nan 1 ; Wang, Meng 2 ; Hou, Yiling 1 ; Zeng, Yichun 3 ; Liu, Gang 3 ; Ding, Xiang 1 ; Huang, Gaiqun 3 ;
作者机构： 1.China West Normal Univ, Coll Life Sci, Key Lab Southwest China Wildlife Resources Conser, 1 Shida Rd, Nanchong 637009, Sichuan, Peoples R China
2.Zhengzhou Univ, Dept Emergency Med, Childrens Hosp, 33 Longhu Outer Ring Rd, Zhengzhou 450000, Henan, Peoples R China
3.Sichuan Acad Agr Sci, Sericulture Res Inst, Nanchong 637000, Sichuan, Peoples R China
关键词： Mulberry; ISSR; SRAP; Genetic diversity; Cluster analysis
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）
ISSN： 1560-8530
年卷期： 2019 年 22 卷 3 期
页码：
收录情况： SCI
摘要： Mulberry, a kind of Moms (Moraceae), is an important perennial economic plant, with leaves used to feed silkworm. It is important to reveal the relationship between the genetic variation and evolution of mulberry and to reveal the phylogenetic of its germplasm. In the present study, the genetic diversity of the gene structure characteristics of 23 main mulberry in Sichuan Province (China) based on inter-simple sequence related (ISSR), sequence-related amplified polymorphism (SRAP) markers and sequencing analysis was reported. 12 ISSRs and 5 SRAPs were exploited, chosen to evaluate the polymorphism level and the discriminating capacity from 100 ISSR primers and 50 SRAP primer. The 12 ISSRs generated 116 repeatable amplified bands, of which 93 (80.24%) were polymorphic. The amplified fragment size was between 0.1-1.5 KB. The 5 SRAP primers produced 31 repeatable amplified bands, of which 24 (77.42%) were polymorphic. The amplified fragment size was also between 0.1-1.5 KB. The study indicated that, ISSR and SRAP markers were reliable and effective to assess the genetic relationship and genetic diversity of 23 main mulberry varieties of Sichuan Province (China). The results provided the scientific basis for the establishment and identification of DNA fingerprint of mulberry varieties germplasm resources. (C) 2019 Friends Science Publishers
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