SIARF10, an auxin response factor, is involved in chlorophyll and sugar accumulation during tomato fruit development
文献类型： 外文期刊
作者： Yuan, Yujin 1 ; Mei, Lihua 1 ; Wu, Mengbo 1 ; Wei, Wei 2 ; Shan, Wei 2 ; Gong, Zehao 1 ; Zhang, Qian 3 ; Yang, Fengqing 3 ;
作者机构： 1.Chongqing Univ, Sch Life Sci, Chongqing 400044, Peoples R China
2.South China Agr Univ, Coll Hort, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangdong Prov Key Lab Postharvest Sci Fruits & V, Guangzhou 510642, Guangdong, Peoples R China
3.Chongqing Univ, Sch Chem & Chem Engn, Chongqing 400044, Peoples R China
4.Sichuan Acad Agr Sci, Hort Res Inst, Chengdu 610066, Sichuan, Peoples R China
关键词： ARF10; auxin; chlorophyll; fruit; starch; sugar; tomato
期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）
ISSN： 0022-0957
年卷期： 2018 年 69 卷 22 期
页码：
收录情况： SCI
摘要： The photosynthesis of green tomatoes contributes to fruit growth and carbon economy. The tomato auxin response factor 10 (SIARF10) belongs to the ARF family and is located in nucleus. In this study, we found that SIARF10 was highly expressed in green fruit. Overexpression of SIARF10 in fruit produced a dark-green phenotype whilst knockdown by RNAi produced a light-green phenotype. Autofluorescence and chlorophyll content analyses confirmed the phenotypes, which indicated that SIARF10 plays an important role in chlorophyll accumulation. Overexpression of SIARF10 positively affected photosynthesis in both leaves and fruit. Furthermore, SIARFIO-overexpression lines displayed improved accumulation of starch, fructose, and sucrose in fruit, whilst SIARFIO-RNAi lines showed decreased accumulation of starch and sucrose. Regulation of SIARF10 expression altered the expression of AGPase starch biosynthesis genes. SIARF10 positively regulated the expression of SIGLK1, POR, CBP1, and CBP2, which are related to chlorophyll metabolism and regulation. Electrophoretic mobility shift assays confirmed that SIARF10 directly targets to the SIGLK1 promoter. Our results thus indicate that SIARF10 is involved in chlorophyll accumulation by transcriptional activation of SIGLK1 expression in tomato fruit, and provide insights into the link between auxin signaling, chloroplast activity, and sugar metabolism during tomato fruit development.
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