您好，欢迎访问四川省农业科学院 机构知识库！

Bi-functional transcription factor SlbHLH95 regulates fruits flavonoid metabolism and grey mould resistance in tomato

文献类型： 外文期刊

作者： Su, Dan 1 ; Wu, Mengbo 1 ; Wang, Hsihua 1 ; Shu, Peng 1 ; Song, Haiyan 3 ; Deng, Heng 4 ; Yu, Shizhe 1 ; Garcia-Caparros, Pedro 5 ; Bouzayen, Mondher 6 ; Zhang, Yang 1 ; Liu, Mingchun 1 ;

作者机构： 1.Sichuan Univ, Coll Life Sci, Key Lab Bioresource Ecoenvironm, Minist Educ, Chengdu, Sichuan, Peoples R China

2.Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China

3.Sichuan Acad Agr Sci, Hort Res Inst, Chengdu, Peoples R China

4.Southwest Univ Sci & Technl, Sch Life Sci & Engn, Mianyang, Peoples R China

5.Univ Almeria, Higher Engn Sch, Almeria, Spain

6.Univ Toulouse, Lab Rech Sci Vegetales Genomique & Biotechnol Frui, CNRS, UPS, Toulouse, France

关键词： tomato; SlbHLH95; bi-functional; flavonoid; fruit quality; SlMYB12

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）

ISSN： 1467-7644

年卷期： 2025 年 23 卷 6 期

页码：

收录情况： SCI

摘要： Flavonoids are polyphenolic secondary metabolites in tomato fruit with important roles in nutritional quality. Dissecting the transcriptional regulatory network modulating flavonoid metabolism is the first step to improve the nutritional quality of tomato fruits through molecular breeding technology. In this study, we identified a transcription factor SlbHLH95 as a key regulator in flavonoid metabolism through analysis of the MicroTom Metabolic Network (MMN) data set. Functional analyses revealed that knockout of SlbHLH95 increased the accumulation of naringenin, while the levels of rutin and nictoflorin decreased. Conversely, overexpression of SlbHLH95 resulted in an opposite pattern of accumulation of flavonoids. Transactivation assays showed that SlbHLH95 positively activated the expression of SlF3H and SlFLS, two key enzyme-encoding genes in the flavonoid pathway, while repressing the expression of SlCHS1. Electrophoretic mobility shift assays (EMSA) demonstrated that SlbHLH95 could directly bind to the promoters of SlF3H and SlFLS, although it could not bind to the promoter of SlCHS1. Furthermore, SlbHLH95 interacted with the transcription factor SlMYB12 and coordinately regulated the expression of SlF3H and SlFLS. Beyond its role in flavonoid metabolism, SlbHLH95 positively regulated the grey mould resistance in tomato fruits by repressing SlBG10. Overall, our findings revealed the important role of bi-functional SlbHLH95 in flavonoid metabolism and grey mould resistance in tomato fruits by acting as both a transcriptional activator and a repressor. This study provides new insights into strategies for improving fruit quality and enhancing fruit disease resistance through targeted genetic modulation.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：四川省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台