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Identification and genetic mapping for rht-DM, a dominant dwarfing gene in mutant semi-dwarf maize using QTL-seq approach

文献类型： 外文期刊

作者： Chen, Qian 1 ; Song, Jun 1 ; Du, Wen-Ping 1 ; Xu, Li-Yuan 1 ; Jiang, Yun 1 ; Zhang, Jie 1 ; Xiang, Xiao-Li 1 ; Yu, Gui-Ro 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol, Chengdu 610061, Sichuan, Peoples R China

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

ISSN： 1976-9571

年卷期： 2018 年 40 卷 10 期

页码：

收录情况： SCI

摘要： Semi-dwarfism is an agronomically important trait in breeding for stable high yields and for resistance to damage by wind and rain (lodging resistance). Many QTLs and genes causing dwarf phenotype have been found in maize. However, because of the yield loss associated with these QTLs and genes, they have been difficult to use in breeding for dwarf stature in maize. Therefore, it is important to find the new dwarfing genes or materials without undesirable characters. The objectives of this study were: (1) to figure out the inheritance of semi-dwarfism in mutants; (2) mapping dwarfing gene or QTL. Maize inbred lines '18599' and 'DM173', which is the dwarf mutant derived from the maize inbred line '173' through Co-60-gamma ray irradiation. F-2 and BC1F1 population were used for genetic analysis. Whole genome resequencing-based technology (QTL-seq) were performed to map dwarfing gene and figured out the SNP markers in predicted region using dwarf bulk and tall bulk from F-2 population. Based on the polymorphic SNP markers from QTL-seq, we were fine-mapping the dwarfing gene using F-2 population. In F-2 population, 398 were dwarf plants and 135 were tall plants. Results of chi(2) tests indicated that the ratio of dwarf plants to tall plants was fitted to 3:1 ratio. Furthermore, the chi(2) tests of BC1F1 population showed that the ratio was fitted to 1:1 ratio. Based on QTL-seq, the dwarfing gene was located at the region from 111.07 to 124.56 Mb of chromosome 9, and we named it rht-DM. Using traditional QTL mapping with SNP markers, the rht-DM was narrowed down to 400 kb region between SNP-21 and SNP-24. The two SNPs were located at 0.43 and 0.11 cM. Segregation analysis of F-2 and BC1F1 indicated that the dwarfing gene was likely a dominant gene. This dwarfing gene was located in the region between 115.02 and 115.42Mb on chromosome 9.

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