文献类型： 外文期刊

作者： Liu, Bin ¹ ; Yao, Lin ² ; Wang, Wenguo ¹ ; Gao, Jihai ¹ ; Chen, Fang ¹ ; Wang, Shenghua ¹ ; Xu, Ying ¹ ; Tang, Lin ¹ ; Jia, ¹ ;

作者机构： 1.Sichuan Univ, Coll Life Sci, Chengdu 610065, Peoples R China

2.Sichuan Acad Agr, Inst Plant Protect, Chengdu 610066, Peoples R China

关键词： senescence;environmental stress;drought effect

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Phospholipase D (PLD, EC 3.1.4.4) is a key enzyme involved in phospholipid catabolism, initiating a lipolytic cascade in membrane deterioration during senescence and stress, which was cloned from Jatropha curcas L., an important plant species as its seed is the raw material for biodiesels. The cDNA was 2,886 bp in length with a complete open reading frame of 2,427 bp which encoded a polypeptide of 808 amino acids including a putative signal peptide of 53 amino acid residues and a mature protein of 755 amino acids with a predicted molecular mass of 86 kD and a pI of 5.44, having two highly conserved 'HKD' motifs. Phylogenetic analysis indicated the J. curcas PLD alpha (JcPLD alpha) showed a high similarity to other PLD alpha from plants. Semi-quantitative RT-PCR analysis revealed that it was especially abundant in root, stem, leaf, endosperm and flower, weakly in seed. And the JcPLD alpha was increasedly expressed in leaf undergoing environmental stress such as salt (300 mM NaCl), drought (30% PEG), cold (4A degrees C) and heat (50A degrees C). The JcPLD alpha protein was successfully expressed in Escherichia coli and showed high enzymatic activities. Maximal activity was at pH 8 and 60A degrees C.