Rapid determination of momordicoside in Momoradica charantia seeds by capillary zone electrophoresis
文献类型： 外文期刊
作者： Xuan, Pu 2 ; Chen, Xin 3 ; Sun, Qun 1 ; Liang, Jia-bei 3 ; Chen, Fang 1 ;
作者机构： 1.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
2.Sichuan Acad Agr Sci, Inst Biol & Nucl Technol, Chengdu 610066, Peoples R China
3.Univ Tradit Chinese Med Chengdu, Coll Pharm, Chengdu 610075, Peoples R China
关键词： Momoradica charantia L.;Momordicoside;Capillary zone electrophoresis;HPLC
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）
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收录情况： SCI
摘要： A novel method is described for the rapid determination of momordicoside in bitter melon based on capillary zone electrophoresis. Electrophoresis conditions, including the pH value and concentration of running buffer, as well as the voltage applied were optimized. The contents of momordicoside in 52 samples originating from different agricultural areas in China were determined. The results showed an excellent linearity in the concentration range of 0.50-2.51 mg mL~(-1) for all samples (R~2>0.9999), with a recovery of 96.0% and a relative standard deviation of 1.4%. The method is characterized by rapidity, accuracy, the need for small samples only, and is suitable for analyses at a large scale.
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